Influence of H2S in the immune-inflammatory answer and in the of periodontal bone loss: study in rats

• Grant number: 16/24094-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2017
• End date: March 31, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Daiane Cristina Peruzzo
• Grantee: Ana Júlia Schmidt Niederauer
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Halitosis is mainly related to the presence of volatile sulphur compounds (VSCs), that include hydrogen sulphide (H2S), methyl mercaptan (CH3SH) and dimethyl sulphide (CH3)2S. Studies showed that the VSCs could be directly involved in the pathogenesis of periodontal disease, however this association is still controversial. Thus, the goal of this study is to evaluate the acute and chronic effect of H2S, in the response of the immune-inflammatory and in the alveolar bone loss in rats, with and without induced periodontal disease. For this subject, 60 rats will be separated in two groups (test - 30 rats will be submitted to daily exposition to H2S, 6 times per day; and control - no exposition) and each group will be subdivided in 3 (n=10), according with the euthanasia time (3 h, 3 days and 14 days). Every animal will have one of their first molars (chosen randomly) submitted to the placement of a bandage for the development of the periodontal disease, in a split-mouth design. After the euthanasia of the animals, it will be collected the marginal portion of the gum around the teeth with bandage and its respective contralateral, for later evaluation of the genes expression through the PCR real time. It will be analyzed the genes expression related to the immune-inflammatory answer and to the bone metabolism: Interleukin-1² (IL-1 ²), Interleukin- 6 (IL-6), RANKL (Receptor activator of nuclear factor kappa-² ligand), OPG (osteoprotegerin), and SOFAT (secreted osteoclastogenic factor of activated T cells); ICTP (pyridinoline cross-linked carboxyterminal telopeptide of type I collagen); and TNF-± (tumor necrosis factor). After the gingival tissue removal, the jaw will be removed, stained and fixed for later morphometric analyses. After the collect of the data the results will be evaluated in relation of their distribution through the Shapiro-Wilks' test and the equivalence of the variants will be tested through the Levene's test. According with the distribution of the data normality, it will be maded parametric or non-parametric tests. For all the tests will be adopted a 5% significance level. (AU)