Characterization of equine amniotic mesenchymal cells after exposure to monocytes

• Grant number: 17/00619-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2017
• End date: March 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Letícia Ribeiro Padoveze
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

This project aims to search for new sources of stem cells, this research presents a study on the collection, isolation and culture of amniotic mesenchymal cells from pre and post-monocyte exposed horses, in order to characterize them in their pluripotency potential, a fundamental characteristic for cells used in cellular therapy. Equine amniotic cells present as an efficient source of autologous stem cells, since in most cases the placenta is discarded after parturition. The collection of the material does not require invasive methods, therefore it presents no risk to the mare or neonate. The collection will be made at the time of parturition, so the samples will be used to characterize the isolation and cell culture. From there, cells will undergo differentiation and cryopreservation protocols. The results will be analyzed in order to obtain greater clarity in the characterization of the protocols submitted to the cell culture of equine amniotic mesenchymal cells. (AU)