Comparison of plasma protein profile of seminal buffalo (Bubalus bubalis) and cattle (Bos indicus)

• Grant number: 16/00603-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2017
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eunice Oba
• Grantee: Viviane Maria Codognoto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The importance of the buffalo species is on their production skills (meat, milk, leather, horn, etc.) and its tensile strength, leading the notoriety of the species in the global economy and investments in similar technology programs to those applied in cattle. So the search for better reproductive rates is leading to selection of genetically superior animals through reproductive biotechnologies, one of the main artificial insemination, which is associated with biotechnology semen. Studies seeking fertility indicators semen making the study of proteins in the seminal plasma object of research in order to identify the proteins that make up the seminal plasma of cattle and buffalos that function as fertility markers. Among these highlight the bovine seminal plasma proteins (BSP) and osteopontin, among others. The aim of this study is to identify the different proteins in the seminal plasma of cattle and buffaloes, determining the relationship and effects of these macromolecules on the basic processes of sperm function. Will be compared semen parameters and the verification and quantification of the presence of proteins in the seminal plasma of buffalo and adult cattle, besides the realization of two-dimensional electrophoresis coupled to mass spectrometry, enabling the development of new techniques that contribute to the increase and prediction fertility and buffalo semen. Keywords: fertility markers, cattle, buffalo, seminal plasma. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CODOGNOTO, VIVIANE MARIA; YAMADA, PAULO HENRIQUE; SCHMITH, RUBIA ALVES; RYDYGIER DE RUEDIGER, FELIPE; DE PAULA FREITAS-DELL'AQUA, CAMILA; DE SOUZA, FABIANA FERREIRA; BROCHINE, SUZANE; DO CARMO, LUCAS MONTEIRO; VIEIRA, ANDRESSA FILAZ; OBA, EUNICE. Cross comparison of seminal plasma proteins from cattle and buffalo (Bubalus bubalis). REPRODUCTION IN DOMESTIC ANIMALS, v. 55, n. 1, p. 81-92, JAN 2020. (16/00603-5)

CODOGNOTO, VIVIANE MARIA; YAMADA, PAULO HENRIQUE; SCHMITH, RUBIA ALVES; DE RUEDIGER, FELIPE RYDYGIER; SCOTT, CAROLINE; LAINETTI, PATRICIA DE FARIA; BROCHINE, SUZANE; FREITAS-DELL'AQUA, CAMILA DE PAULA; DE SOUZA, FABIANA FERREIRA; OBA, EUNICE. Functional insights into the role of seminal plasma proteins on sperm motility of buffalo. Animal Reproduction Science, v. 195, p. 251-258, AUG 2018. (16/00603-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CODOGNOTO, Viviane Maria. Perfil proteico do plasma seminal de búfalos (bubalus bubalis) e bovinos (bos taurus indicus). 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.