Development of a technique combining photobiomodulation and radiotherapy to enhance the tumoral response to ionizing radiation

• Grant number: 17/14182-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2017
• End date: April 30, 2021
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Clara Maria Gonçalves de Faria
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

Photobiomodulation (PBM), a technique that is being used for over 40 years, is based on applying light in order to modify cellular processes such as proliferation, tissue regeneration and analgesia. These effects are strong evidences that PBM can modulate the cell cycle and increase tissue oxygenation, which are determinant factors in a radiotherapy result. Therefore, in this project it is proposed the investigation of the combination of these techniques to the deleopment of a protocol that enhances tumor damage and treatment efficacy. In vitro experiments will enable the study of PBM in cell proliferation and cell cycle and its combination with radiotherapy, using the MTT assay and flow cytometry. From these experiments it will be determined the protocols to be analyzed in vivo. It will be used an oral cavity cancer model, using the human squamous cell carcinoma cells SCC-25, and in vivo tumor induction will be performed in hairless mice. The aim of this experiments is the evaluation of the techniques combination in a model of higher biological complexity and the analysis of tumor damage and animal survival in order to propose a protocol that enhances the cancer response to ionizing radiation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES DE FARIA, CLARA MARIA; CIOL, HELOISA; BAGNATO, VANDERLEI SALVADOR; PRATAVIEIRA, SEBASTIAO. Effects of photobiomodulation on the redox state of healthy and cancer cells. BIOMEDICAL OPTICS EXPRESS, v. 12, n. 7, p. 3902-3916, JUL 1 2021. (14/50857-8, 09/54035-4, 13/07276-1, 17/14182-4)

DE FARIA, CLARA M. G.; COSTA, CAMILLA S.; BAGNATO, VANDERLEI S.. Photobiomodulation effects on photodynamic therapy in HNSCC cell lines. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 217, APR 2021. (14/50857-8, 13/07276-1, 17/14182-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FARIA, Clara Maria Gonçalves de. Photobiomodulation effects and applications in oncology. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.