Study of c-di-GMP signalling mechanism in Leptospira interrogans serovar Copenhageni by LIC_11129

• Grant number: 17/10611-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2017
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Cristiane Rodrigues Guzzo Carvalho
• Grantee: Aline Dias da Purificação
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Leptospirosis is a disease of broad incidence in tropical countries and your transmission is caused by species of Leptospira genus, and have resistant to environmental changes, such as temperature and pH. It's believed that this resistance is in somehow related to the process of regulation of the second bacterial messenger c-di-GMP by DGC activity in proteins with GGDEF domains. In this project, the mechanism of c-di-GMP signaling in Leptospira interrogans serovar Copenhageni mediated by LIC_11129, which has a GGDEF domain at C-terminal portion and a PAS domain at the N-terminal, will be studied in order to verify the hypothesis of the regulatory role of PAS domain upon GGDEF in each protein. For this, we intend to solve the structure of the PAS domain, predict its possible ligand and verify how the effector regulates the DGC activity of each protein. The role of c-di-GMP in Leptospira is not very well known, our group is the first to show the production of this dinucleotide in Leptospira. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALINE DIAS DA, PURIFICACAO; NATHALIA MARINS DE, AZEVEDO; GABRIEL GUARANY DE, ARAUJO; ROBSON FRANCISCO DE, SOUZA; RODRIGUES, GUZZO CRISTIANE. The World of Cyclic Dinucleotides in Bacterial Behavior. Molecules, v. 25, n. 10, MAY 2020. (18/21076-9, 16/08414-7, 17/10611-8, 18/08996-1, 19/00195-2)