Effects of mechanical vibration on inflammatory cytokines in keratinocytes challenged by lipopolysaccharide of Porphyromonas gingivalis

• Grant number: 17/12079-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2017
• End date: November 30, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Lia Anbinder
• Grantee: Luma Collino
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Periodontitis is an infectious-inflammatory disease that affects the periodontal tissues. Its etiology includes Gram-negative bacteria like Porphyromonas gingivalis. Alternative and adjunct treatments for supra and subgingival scaling have been studied, and the search for less traumatic and invasive therapies is increasing. The use of oscillatory mechanical signals of low intensity (vibrations) has shown satisfactory results in the tissue repair, through the increase of the expression of pro-healing growth factors and decrease of pro-inflammatory cytokines production, in the reduction of osteoclastogenesis and bone resorption. Due to the positive results regarding bone remodeling, extraoral vibratory devices were launched in the market aiming to accelerate orthodontic treatment. Acceledent® (Ortho Accel Technologies, Inc., Bellaire, TX) that works by applying a cyclic force of 0.25N (25g) at the frequency of 30 Hz, and should be used for 20 minutes is one of these devices. Although it is already available in the market, the effect it causes in periodontal disease or bacteria present in the oral microbiota has not been found in the literature. The objective of this work is to evaluate the effects of vibration on lipopolysaccharide-challenged keratinocytes (LPS) of Porphyromonas gingivalis, simulating periodontal disease in vitro. Human immortalized keratinocytes (HaCaT) challenged by LPS (or PBS) will be cultured. Following the addition of LPS (or PBS), the plates will receive the vibration protocol (30 Hz for 20 min) and two hours after the initiation of the vibration, the cell viability will be assessed and also the supernatant collected for further quantification of IL-1² and TNF-±. The same vibration procedure will be repeated 24 h and 48 h after addition of LPS / PBS, and the analyzes will occur 2 h after the first and third vibration sessions. The data will be submitted to the most convenient statistical test, at the 5% level. (AU)