On the Artepillin C effects over the in vitro culture of cells derived from oropharyngeal carcinoma (HEp-2)

• Grant number: 18/02392-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2018
• End date: November 30, 2018
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Pedro Henrique Benites Aoki
• Grantee: Mirella Boaro Kobal
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

Green própolis has received considerable attention due to the multiple applications, including therapeutic properties against the proliferation of tumor cells. Artepillin C is the major component of própolis and its antitumor activity have been the focus of many studies. However, the incorporation effects of Artepillin C into the culture of cancer cells have not yet been explored. Herein, we shall investigate the cellular cytotoxicity of Artepillin C in cells derived from the oropharyngeal carcinoma (HEp-2). Cytotoxicity tests will be performed with the addition of different concentrations (10-3 to 10-6 mol/L) of Artepillin C, diluted in methanol and F12 DMEM culture medium, into the in vitro culture of HEp-2 cells. The influence of different incubation times (12, 24 and 48h) on the cell culture will also be evaluated in order to ensure its therapeutic use. In addition, broad-field and confocal fluorescence microscopies will be performed to analyze the cellular morphology and flow cytometry to determine the cellular death processes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOBAL, MIRELLA B.; PAZIN, WALLANCE M.; BISTAFFA, MARIA J.; CONSTANTINO, CARLOS J. L.; TOLEDO, KARINA A.; AOKI, PEDRO H. B.. Correlating Artepillin C cytotoxic activity on HEp-2 cells with bioinspired systems of plasma membranes. Materials Science & Engineering C-Materials for Biological Applications, v. 112, JUL 2020. (18/02392-7, 16/09633-4)