Genetic and molecular studies in Panicum maximum: genetic mapping with SNP markers based on Genotyping-by-Sequencing

• Grant number: 17/17969-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2018
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Anete Pereira de Souza
• Grantee: Thamiris Gatti Deo
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Panicum maximum is the most productive tropical forage at the national level and is responsible for a large part of beef cattle fattening in Brazil and in several Latin American countries. In spite of the great economic importance, researches related to the genetic of specie are scarce, mainly because of high complexity of the genome and ploidy level (4n), besides the low investment for the research in forage grasses. In this context, the project aims to contribute to the genetic and molecular knowledge of P. maximum, by increasing the resolution of a genetic linkage map constructed previously using a base population of high importance for the breeding program of specie. Therefore, the SNPs markers (Single Nucleotide Polymorphism) will be detected from the Genotyping-by-Sequencing (GBS), that allows the genotyping of thousands of markers quickly and with low cost , and will be inserted in the previous map. The results obtained will contribute to a better molecular genetic knowledge of P. maximum and will be the basis for other studies of the species and other forage grasses. From these results, it will be possible identify genes and molecular markers related to characteristics of important agronomic/economical that in the long term, may support the Marker Assisted Selection (SAM) in breeding programs of this forage grass. (AU)