Effects of exosomes derived from benign myoepithelial cells on the invasion of human malignant epithelial cells in Matrigel

• Grant number: 17/15458-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2018
• End date: April 30, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Lucas Novaes Teixeira
• Grantee: Giuseppe Zangrandi Coppola
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Cell communication, either in physiological or pathological conditions, is mediated mainly by molecules present in cellular surface as well as via soluble factors secreted by cells. Recently, a great interest has emerged regarding the mechanism of cell-to-cell communication through vesicles called exosomes. These structures are produced by many different cell types and can modulate cellular activity by induction of epigenetic alterations. Among the biological mechanisms modulated by exosomes, the tumor invasion process has been highlighted in recent studies. In this context, the present study aims to evaluate the in vitro effect of benign myoepithelial cell derived exosomes on the invasion of malignant neoplastic epithelial cells. For this purpose, we propose: 1) isolate and quantify exosomes derived from benign myoepithelial cells; 2) analyze a gene expression of metalloproteases 2 and 9 in malignant epithelial cell cultures exposed to the exosomes derived from benign myoepithelial cells; and 3) quantify the invasion of neoplastic malignant epithelial cells in Matrigel after exposure to exosome derived from benign myoepithelial cells.