Characterization of the stem cell niche in the gastric mucosa of developing and adult rats and the role of early weaning on this population

• Grant number: 18/05064-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: June 01, 2018
• End date: February 28, 2023
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Patrícia Gama
• Grantee: Isadora Campos Rattes
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The gastric body is the main region responsible for the digestive process, and has thick mucosa formed by tubular glands that open in pits on the surface. In adult individuals, these glands are divided into three segments: isthmus, colon and base and different epithelial populations are present. Stem cells are located between the isthmus and the neck region of the adult gland, but during postnatal development this niche is not yet determined, and proliferative cells are distributed throughout the gland. Despite the various studies and the great debate about the characterization of the gastric stem cell, it is not known which cell markers are present and the organization of the microenvironment, in terms of growth and signaling factors, is unknown. In addition, since the dietary pattern can alter the distribution of proliferative cells, it is necessary understand if and how the determinants of the stem-cell niche are influenced by diet. Thus, considering that breastfeeding plays an important role in the development of the gastric mucosa, early weaning (a common social practice) can lead to diverse changes, and also affect the gastric niche compartment. Our proposal aims is to evaluate the elements that form and establish the niches of stem cells and that determine their proliferation and migration, both in puppies and in adults, and to observe the immediate and late effects of early weaning in this population. During postnatal development, Wistar rats will be submitted to early weaning and the stomach will be collected at different ages for experiments in evaluation of gene expression, cell migration, detection and localization of molecules important for niche establishment. The obtained results will be grouped and analyzed statistically. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARRETO E BARRETO, LAYLLA; RATTES, ISADORA C.; DA COSTA, ALINE V.; GAMA, PATRICIA. Paneth cells and their multiple functions. Cell Biology International, v. 46, n. 5, p. 10-pg., 2022-01-23. (18/05064-0, 18/07409-5, 18/07782-8)

MESQUITA DA SILVA, KETHLEEN; RATTES, ISADORA CAMPOS; PEREIRA, GIZELA MARIA AGOSTINI; GAMA, PATRICIA. Lifelong Adaptation of Gastric Cell Proliferation and Mucosa Structure to Early Weaning-Induced Effects. FRONTIERS IN PHYSIOLOGY, v. 12, SEP 13 2021. (14/21449-9, 18/05064-0, 18/07782-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RATTES, Isadora Campos. Characterization of proliferative cells in developing and adult rats after early weaning.. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.