Effect of early weaning on molecular markers and development of intestinal mucosa

• Grant number: 18/07409-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2018
• End date: September 30, 2021
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Patrícia Gama
• Grantee: Aline Vasques da Costa
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The main functions of the gastrointestinal tract are the digestion and absorption of nutrients mediated by specialized cells of the epithelial tissue. The activity of development, renewal and regeneration is controlled by the niche of intestinal stem cells located at the base of the crypts, however it is still unknown how this niche of proliferative cells behaves in face of alterations in dietetic pattern. Considering breastfeeding as an important role in development of intestinal mucosa, early weaning may promote changes in the process of cell differentiation and proliferation. Recent studies have analyzed epigenetic patterns of intestinal epithelial cells and demonstrated that the plasticity of the cell is maintained by a permissive epigenomic state and the specification of cell line is directed by activation of signaling pathways and expression of factors and transcription. Therefore, the aim of this proposal is evaluate markers related to the process of cell proliferation, differentiation and growth factors of the intestinal epithelium as well as possible epigenetic variation observing the immediate and late effects of early weaning. During postnatal development, Wistar rats will be submitted to early weaning and gut will be collected at different ages for evaluation of gene and protein expression, detection and localization of important molecules for the establishment of the niche. The obtained results will be grouped and analyzed statistically. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA COSTA, ALINE VASQUES; GOES, CAROLINA PURCELL; GAMA, PATRICIA. Breastfeeding importance and its therapeutic potential against SARS-CoV-2. PHYSIOLOGICAL REPORTS, v. 9, n. 3, FEB 2021. (20/02888-2, 18/07782-8, 18/07409-5)

BARRETO E BARRETO, LAYLLA; RATTES, ISADORA C.; DA COSTA, ALINE V.; GAMA, PATRICIA. Paneth cells and their multiple functions. Cell Biology International, v. 46, n. 5, p. 10-pg., 2022-01-23. (18/05064-0, 18/07409-5, 18/07782-8)

DA COSTA, ALINE VASQUES; RATTES, ISADORA CAMPOS; GOES, CAROLINA PURCELL; LOBO, LARISSA HONDA GRECO; BARRETO, LAYLLA BARRETO E; GAMA, PATRICIA. Breastfeeding lifespan control of growth, maintenance, and metabolism of small intestinal epithelium. Journal of Cellular Physiology, v. N/A, p. 12-pg., 2023-08-09. (18/07409-5, 18/07782-8)

DA COSTA, ALINE VASQUES; RATTES, ISADORA CAMPOS; SILVA, KETHLEEN MESQUITA; MENDOZA RIGONATI, CRUZ ALBERTO; GAMA, PATRICIA. Effect of early weaning on proliferation and differentiation of intestinal mucosa. FASEB JOURNAL, v. 33, p. 2-pg., 2019-04-01. (18/07409-5, 18/07782-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COSTA, Aline Vasques da. Effect of early weaning on molecular markers and intestinal mucosal development.. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.