| Grant number: | 18/07766-2 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | August 01, 2018 |
| End date: | September 30, 2020 |
| Field of knowledge: | Biological Sciences - Biochemistry - Biochemistry of Microorganisms |
| Principal Investigator: | Sergio Schenkman |
| Grantee: | Gregory Pedroso dos Santos |
| Host Institution: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil |
| Associated research grant: | 15/22031-0 - Cell signaling in Trypanosoma during host-parasite interaction, AP.TEM |
Abstract The present PhD project, entitled "Characterization of TcCyp19 and evaluation of its role during the cellular infection by Trypanosoma cruzi", was initiated by the joint work of Prof. Sergio Schenkman with Prof. Bradford Mcgwire of Ohio University who initiated studies showing the expression of cyclophilin 19 (TcCyp 19) in T. cruzi, the protozoan parasite that causes Chagas' disease. They verified that the TcCyp 19 is secreted by the replicative forms found in the insect triatomine vector being able to inactivate an antimicrobial peptide and that in the inactive form causes the signaling of the calcineurin in the parasites, leading to a greater infectivity. It has also been found that TcCyp19 is acetylated on the side chain of various lysines. Cyclophilins compose a family of very well conserved proteins between organisms. TcCyp 19 has great similarity with the cyclophilin A (Cyp A) found in mammals that acts among others in the cellular processes of inflammation in cardiac cells. In this research project we aim to study the role of TcCyp 19 during T. cruzi infection in mammalian cells in order to verify this protein could have some role in the inflammatory events of Chagas disease. More specifically we intend to: 1) determine the amounts of TcCyp19 expressed in the different stages of the parasite; 2) to verify whether it is secreted into the extracellular medium and into the cytosol of infected mammalian cells; 3) investigate the mechanisms involved, possibly acetylation, for its secretion; 4) to verify whether the presence of TcCyp19 in the cytosol or in the culture medium in some way affects survival, growth when the host cell is subjected to stresses that induce cell death. (AU) | |
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