Effect of systemic administration of lycopene on bone repair in calvaria of ovariectomized rats

• Grant number: 17/20407-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Selma Siéssere
• Grantee: Vitória Ricardo
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Osteoporosis is one of the leading causes of mortality and morbidity around the world. This disease is characterized by the deterioration of the bone microarchitecture, causing its fragility and increasing the risk of fractures. The prevalence of osteoporosis has been increased in view of the raise in life expectation of the population. Although estrogen deficiency has been considered the most important mechanism for osteoporosis, studies indicate that reactive oxygen species are the major factors for this disease. Some measures can reduce fracture levels and raise bone mass indexes, such as physical activity and a balanced diet with nutrient richness. The ingestion of carotenoid-containing foods has been indicated in the sense of preventing bone loss. Lycopene, a carotenoid found in tomatoes and other vegetables, is a potent antioxidant capable of stimulating cell proliferation, inhibiting the formation of osteoclasts and bone resorption. This study aims to verify the efficiency of lycopene intake in the bone repair process in defects created in calvaria of ovariectomized rats. Lycopene will be administered by means of a gastric tube at a concentration of 45 mg / kg body weight. After 60 days of ovariectomy surgery, bone defects of 5 mm will be made and after 31 days, the animals will be sacrificed. Bone fragments will be collected and fixed for later histological processing. The obtained sections will be stained in Masson's Trichrome and Hematoxylin - Eosin for the morphological and quantitative analyzes, respectively. The latter will be done using the Image J software. The animals will be divided into the following groups: control / sham (C); ovariectomized (OVX); ovariectomized + lycopene administered immediately after ovariectomy until euthanasia (OVXL). Data will be submitted for analysis using the GraphPad Prism® statistical program, and the significance will be set at 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RICARDO, V.; SOUSA, L. G.; SCALIZE, P. H.; REGALO, I. H.; PITOL, D. L.; BOMBONATO-PRADO, K. F.; REGALO, S. C. H.; SIESSERE, S.. LYCOPENE INFLUENCES BODY WEIGHT AND CALVARIA BONE REPAIR IN OVARIECTOMIZED RATS. OSTEOPOROSIS INTERNATIONAL, v. 31, n. SUPPL 1, p. 2-pg., 2020-12-01. (17/20407-9)

VITÓRIA RICARDO; LUIZ GUSTAVO DE SOUSA; ISABELA HALLAK REGALO; DIMITRIUS LEONARDO PITOL; KARINA FITTIPALDI BOMBONATO-PRADO; SIMONE CECILIO HALLAK REGALO; SELMA SIESSERE. Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats. Brazilian Dental Journal, v. 34, n. 3, p. 50-56, 2023-07-17. (17/20407-9)