Analysis of the effect of biomodulation and the surface treatment method by electrolytic plasma oxidation in miniplates of fixation in bone repair of osteoporotic rats

• Grant number: 18/11496-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2018
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Leonardo Perez Faverani
• Grantee: João Matheus Fonseca e Santos
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This project will evaluate the effect of biomodulation and bioactivity on the bone repair of ovariectomized rats submitted to the simulation of femoral fractures. 32 female Wistar rats (Rattus novergicus), at 6 months of age, in which half of the animals will be submitted to bilateral ovariectomy (OVX) and the other half to the fictitious ovariectomy surgery (SHAM), and a period of 3 months will be proserved for the induction of osteoporosis. The rats will be submitted to the fracture simulation in one of the femurs and the fracture will be fixed with miniplate and screws of the system 1,5 mm. Half of the samples will have miniplates with surfaces treated by oxidation with electrolytic plasma (PEO), which will be in contact with the repair gap and the other half with conventional miniplates with machined surface (CONV). While half of the samples will be submitted to biomodulation by low intensity laser (BLLLT) for 5 minutes, the other party will not undergo this process (SB). Euthanasia of all animals will be performed at 60 postoperative days. Euthanasia of all animals will be performed at 60 postoperative days. The experimental groups (SHAM / BLLLT, SHAM / SB, OVX / BLLLT, OVX / SB), after euthanasia, will have the regions of interest initially scanned in computerized microtomography to evaluate bone volume parameters (BV and BV / TV) and bone quality (Tb.Th, Tb.Sp and Tb.N), then the pieces will continue to be processed for inclusion in photopolymerizable resin. At the 14 and 42 postoperative days, calcein and alizarin, respectively, were given intramuscularly at a dose of 20 mg / kg body weight. The slides will be scanned in laser confocal microscopy to calculate the bone turnover, by means of the fluorochrome area and the mineral apposition rate (MAR). After this process, the slides will be stained in alizarin red and Stevenel blue for neoformed bone area histometry in the reparative gap. In the euthanasia will be collected blood of the animals that will be analyzed by immunoassay (ELISA), for the dosage of estrogen between the groups. All the quantitative data will be submitted to the normality curve to establish the best statistical test (parametric or non-parametric), considering p <0.05 the level of significance.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRAGA POLO, TARIK OCON; CORREA MOMESSO, GUSTAVO ANTONIO; PEREIRA SILVA, WILLIAM PHILLIP; DE SOUZA SANTOS, ANDERSON MAIKON; FONSECA-SANTOS, JOAO MATHEUS; DA CRUZ, NILSON CRISTINO; BARAO, VALENTIM A. R.; GARCIA, VALDIR GOUVEIA; THEODORO, LETICIA HELENA; FAVERANI, LEONARDO P.. Is an anodizing coating associated to the photobiomodulation able to optimize bone healing in ovariectomized animal model. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 217, APR 2021. (18/11496-0, 16/20297-6)

POLO, TARIK OCON BRAGA; FONSECA-SANTOS, JOAO MATHEUS; MOMESSO, GUSTAVO ANTONIO CORREA; DA SILVA, WILLIAM PHILLIP PEREIRA; BARBOSA, STEFANY; SANTOS, ANDERSON MAIKON DE SOUZA; SILVA, MIRELA CAROLINE; GARCIA, VALDIR GOUVEIA; THEODORO, LETICIA HELENA; FAVERANI, LEONARDO P.. Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis. Lasers in Medical Science, v. 38, n. 1, p. 10-pg., 2023-03-20. (16/20297-6, 18/11496-0)