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Expression of miRNAs in ocular Toxoplasmosis

Grant number: 18/09448-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2018
Effective date (End): September 16, 2022
Field of knowledge:Biological Sciences - Immunology - Immunogenetics
Principal researcher:Luiz Carlos de Mattos
Grantee:Geraldo Magela de Faria Junior
Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
Associated scholarship(s):19/05581-8 - Techniques of genotyping for molecular epidemiology and population study of Toxoplasma gondii, BE.EP.DR

Abstract

Toxoplasmosis is an endemic disease worldwide and responsible for various morbidities in both humans and animals. It has emerged as one of the most common opportunistic infections in immunocompetent patients and may be fatal to the fetus. Toxoplasma gondii infection, the etiological agent of toxoplasmosis, results in the induction of immune responses (innate and adaptive) with consequent production of cytokines, antibodies and activation of T lymphocytes (CD8, CD4-TH1, CD4-TH2, B). microRNAs (miRNAs) are molecules of non-coding RNAs that have 21-25 nucleotides and function as a negative regulator of gene expression. The overall objective of this project is to investigate microRNA expression levels (miR-712-3p, miR 511-5p, miR-217-5p, miR 9-2) in patients with active lesion and scar resulting from T. infection. gondii. Its specific objectives include: 1- Select three groups so constituted: Group 1 (G1): patients with active ocular lesion and reactive serology; Group 2 (G2): patients with ocular scar and reactive serology; Group (G3): patients without lesion or ocular scar from toxoplasmosis, with reactive serology. 2- To verify the presence of anti-T.gondii antibodies of classes IgM and IgG in the serum of these patients as identifiers of acute and chronic infection; 3- Extract and quantify the miRNAs miR-712-3p, miR 511-5p, miR-217-5p and miR 9-2 in the three groups of patients; 4 - Relate the presence of ocular lesion and its activity as well as the ocular scar with the expression levels of the miRNAs mentioned above. Three groups of patients will be selected at the Hospital de Base Retinopathy Outpatient Clinic (FUNFARME), characterized as follows: Group 1 (G1) at least 100 consecutive ethnically independent patients with active lesion and reactive serology for ocular toxoplasmosis. Group 2 (G2) at least 100 patients who present reactive serology and ocular scar; Group 3 (G3) at least 100 patients with reactive serology and no lesion or ocular scar from toxoplasmosis. For the serological analysis, the ELFA immunoenzymatic test will be performed. For analysis of the expression of specific microRNAs by quantitative Real-Time PCR (qRT-PCR). Quantitative variables will be described by mean, median and standard deviation. To verify the accuracy of the use of the miRNAs for the diagnosis of T.gondii infection will be used the ROC curve. A confidence interval (CI) of 95.0% will be considered and the differences will be considered statistically significant when the p value is d 0.05. The results may contribute to the understanding of the pathogenesis of ocular toxoplasmosis

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOMES, ISABELA BRONCHTEIN; AYO, CHRISTIANE MARIA; LOPES, ALESSANDRO GARCIA; KUMANO, LAURIE SAYURI; DE FARIA JUNIOR, GERALDO MAGELA; DE ALMEIDA JR, GILDASIO CASTELLO; CASTIGLIONI, LILIAN; DE MATTOS, LUIZ CARLOS; BRANDAO, CINARA CASSIA. Influence of interleukin 17 A and 17 F polymorphisms in keratoconus. MOLECULAR BIOLOGY REPORTS, v. 48, n. 11 SEP 2021. Web of Science Citations: 0.
DE FARIA JUNIOR, GERALDO MAGELA; MURATA, FERNANDO HENRIQUE ANTUNES; LORENZI, HERNAN ALEJANDRO; CASTRO, BRUNO BELLO PEDE; ASSONI, LETICIA CAROLINA PARABOLI; AYO, CHRISTIANE MARIA; BRANDAO, CINARA CASSIA; DE MATTOS, LUIZ CARLOS. The Role of microRNAs in the Infection by T. gondii in Humans. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAY 14 2021. Web of Science Citations: 1.
MURATA, FERNANDO HENRIQUE ANTUNES; PREVIATO, MARIANA; FREDERICO, FABIO BATISTA; BARBOSA, AMANDA PIRES; NAKASHIMA, FABIANA; DE FARIA, JR., GERALDO MAGELA; SILVEIRA CARVALHO, APARECIDA PERPETUO; MEIRA STREJEVITCH, CRISTINA DA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; CASTIGLIONI, LILIAN; DE MATTOS, LUIZ CARLOS; SIQUEIRA, RUBENS CAMARGO; BRANDAO DE MATTOS, CINARA CASSIA. Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in Sao Paulo State, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 9, FEB 7 2020. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.