Essential oils of thyme, lemongrass and rosemary in the control of Aspergillus sp. inoculated in seeds of broccoli, arugula, cauliflower and squash

• Grant number: 18/14890-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2019
• End date: December 31, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Pâmela Gomes Nakada Freitas
• Grantee: César Augusto Santos
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil

Abstract

The demand for food has been growing along with the increase in the world population, which stimulates the use of conventional agricultural pesticides. With the aim of reducing negative effects, organic agriculture is an alternative to recommending the use of natural products. However, organic products are still scarce, and in view of the increasing pressure of diseases and pests on modern agriculture, it is essential to study new options to control these problems. In relation to the fungi that are associated with seeds, the genus Aspergillus is the group with the highest contamination in storage. The objective of this study was to evaluate the effect of the essential oils of thyme, lemon balm and rosemary on broccoli, arugula, cauliflower and moringa seeds inoculated with the fungus Aspergillus flavus. There will be four experiments, one of each plant species, seeds of broccoli, arugula, cauliflower and squash, and the treatments will be in a 3 x 5 factorial scheme, will be in total three essential oils: thyme (Thymus vulgaris), lemongrass (Cymbopogon citratus) and rosemary (Rosmarinus officinalis), and five concentrations: 0; 0.4; 0.8; 1.6; 2.0%. The seeds will be treated in an orbital shaker table (model-MA 376) for five minutes. The experimental design will be completely randomized with four replicates. The characteristics to be evaluated will be: germination, first germination count, shoot length, primary root and total seedling length, seedling dry mass, emergency speed index, emergency test and sanity test. In order to evaluate the results, each plant species will be submitted to analysis of variance, and the regression analysis will be applied to 5% probability for the factor concentration, for the essential oils factor, the Tukey test will be applied to 5% of probability.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAKADA-FREITAS, PAMELA G.; SANTOS, CESAR AUGUSTO; MAGALHAES, THALITA HELENA; BUSTAMONTE, STEFANI S.; DOS SANTOS, DANIELLE CARLA; INACIO CARDOSO, ANTONIO ISMAEL; AMADOR, TALITA S.; LANNA, NATALIA DE B. L.; BARDIVIESSO, ESTEFANIA M.; CATAO, HUGO CESAR R. M.. Effect of thyme, lemongrass and rosemary essential oils on Aspergillus flavus in cauliflower seeds. Horticultura Brasileira, v. 40, n. 1, p. 5-pg., 2022-01-01. (18/14890-1)