Defining the role of cementocytes in dental cementum homeostasis during orthodontic movement

• Grant number: 19/09435-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 12, 2019
• End date: August 11, 2020
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Francisco Humberto Nociti Junior
• Grantee: Elis Janaína Lira dos Santos
• Supervisor: Brian Lee Foster
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: Ohio State University, Columbus, United States
• Associated to the scholarship: 18/26341-2 - Defining the role of cementocytes in dental cementum homeostasis during orthodontic movement, BP.DR

Abstract

Cementocytes not only share morphological features with osteocytes, but also express several common biomakers. However, in contrast to osteocytes, there is a lack of evidence on the potential biological properties of cementocytes. Thus, we hypothesized that cementocytes play an important role in dental cementum homeostasis during orthodontic tooth movement (OTM). In order to test our hypothesis, a comparative and systematic proteomic analysis of the dental cementum will be performed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), followed by immunohistochemistry (IHC) to validate LC-MS/MS selected findings in areas whether or not occurred orthodontic tooth movement. In addition, morphological and ultrastructural changes in cementocytes will be assessed by transmission electron microscopy, confocal microscopy in samples labeled with fluorescein isothiocyanate and micro-computed tomography. We anticipate we will be able to define the role of cementocytes in dental cementum homeostasis during OTM and also map potential biomolecules critically involved with dental cementum homeostasis. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, E. J. LIRA; CHAVEZ, M. B.; TAN, M. H.; MOHAMED, F. F.; KOLLI, T. N.; FOSTER, B. L.; LIU, E. S.. Effects of Active Vitamin D or FGF23 Antibody on Hyp Mice Dentoalveolar Tissues. JOURNAL OF DENTAL RESEARCH, v. 100, n. 13, APR 2021. (19/09435-6)

LIRA DOS SANTOS, ELIS J.; SALMON, CRISTIANE R.; CHAVEZ, MICHAEL B.; DE ALMEIDA, AMANDA B.; TAN, MICHELLE H.; CHU, EMILY Y.; SALLUM, ENILSON A.; CASATI, MARCIO Z.; RUIZ, KARINA G. S.; KANTOVITZ, KAMILA R.; et al. Cementocyte alterations associated with experimentally induced cellular cementum apposition in hyp mice. Journal of Periodontology, JUN 2021. (15/06372-2, 19/09435-6)

LIRA DOS SANTOS, ELIS J.; DE ALMEIDA, AMANDA B.; CHAVEZ, MICHAEL B.; SALMON, CRISTIANE R.; MOFATTO, LUCIANA S.; CAMARA-SOUZA, MARIANA BARBOSA; TAN, MICHELLE H.; KOLLI, TAMARA N.; MOHAMED, FATMA F.; CHU, EMILY Y.; et al. Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature. BONE, v. 153, DEC 2021. (18/26341-2, 19/09435-6)

LIRA DOS SANTOS, ELIS J.; SALMON, CRISTIANE R.; CHAVEZ, MICHAEL B.; DE ALMEIDA, AMANDA B.; TAN, MICHELLE H.; CHU, EMILY Y.; SALLUM, ENILSON A.; CASATI, MARCIO Z.; RUIZ, KARINA G. S.; KANTOVITZ, KAMILA R.; et al. Cementocyte alterations associated with experimentally induced cellular cementum apposition in hyp mice. Journal of Periodontology, v. 92, n. 11, p. 116-127, NOV 2021. (15/06372-2, 19/09435-6)