Nitrification inhibitor as strategy to mitigate N2O emissions and its impacts on the active nitrifying and denitrifying soil microbial communities after concentrated vinasse and mineral N application

• Grant number: 18/20698-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2019
• End date: February 28, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Heitor Cantarella
• Grantee: Késia Silva Lourenço
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated scholarship(s): 20/15773-9 - The role of fungal denitrifiers in the nitrous oxide emission from soils with organic residues amendment, BE.EP.PD

Abstract

Nitrogen fertilization has been one of the main sources of greenhouse gas (GHG) emissions to the atmosphere in sugarcane cultivation, mainly due to the emission of nitrous oxide (N2O). Recent studies indicate that the application of vinasse (concentrated-VC and in natura), a byproduct of ethanol production - especially with the addition of nitrogen (N) - results in significant increases in N2O emissions. Alternatives to reduce these emissions are necessary because the main destination of vinasse is the soil, where it recycles nutrients and improves fertility. One way to reduce this emission is to add nitrification inhibitors (IN), but little is known about the efficiency of INs when mineral N is mixed with vinasse and its effect on the soil microbial community. Therefore, the objective of this project is to evaluate the efficiency of IN, 3,4-dimethylpyrazole phosphate (DMPP) in reducing N2O emissions when mineral N is applied together with VC. In addition, the impact of the joint application of IN, VC and N mineral in the total microbial community of the soil (bacteria, archaea and fungus) and in the nitrifying community (bacteria and artery) and denitrifying (bacterium and fungus) will be determined. The strategy will be tested in field experiments setup at the beginning and end of the harvest season (dry and rainy season) and in laboratory experiments. The N and VC doses will be 120 kg ha-1 and 7 m3 ha-1, respectively. Gaseous (CO2, CH4 and N2O) will be intensively sampled, as well as soil sampling for NH4+, NO3-, pH and soil moisture determination. The DNA and RNA of the soil samples will be extracted with subsequent amplification and sequencing of the 16S rRNA genes (bacterium and total archaea) and ITS (total fungus). The genes linked to the N cycle (amoA, hao, nxr, nirK, nirS, norB and nosZ) will be amplified and quantified to determine the processes responsible for the production of N2O. The results obtained can be used to define sustainable management practices during the production of ethanol, as well as to improve the understanding of the microbial processes responsible for the production of N2O and the identification of such microorganisms, and specially to determine the participation of the denitrification fungi in the emissions of N2O in soils with sugarcane and straw on the soil surface.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, BRUNA G.; LOURENCO, KESIA S.; CARVALHO, JOAO LUIS N.; GONZAGA, LEANDRO C.; TEIXEIRA, MARIA CAROLINA; TAMARA, ANA FLAVIA; CANTARELLA, HEITOR. Soil pH does not interfere with nitrification inhibitor efficiency for reducing N2O emissions from soils treated with concentrated vinasse and urea. Geoderma, v. 426, p. 9-pg., 2022-08-10. (17/02299-4, 18/20793-9, 19/12557-6, 18/20698-6)

SULEIMAN, AFNAN KHALIL AHMAD; LOURENCO, KESIA SILVA; CLARK, CALLUM; LUZ, RONILDSON LIMA; RIBEIRO DA SILVA, GUSTAVO HENRIQUE; VET, LOUISE E. M.; CANTARELLA, HEITOR; FERNANDES, V, TANIA; KURAMAE, EIKO EURYA. From toilet to agriculture: Fertilization with microalgal biomass from wastewater impacts the soil and rhizosphere active microbiomes, greenhouse gas emissions and plant growth. RESOURCES CONSERVATION AND RECYCLING, v. 161, OCT 2020. (13/50351-4, 18/20698-6, 14/24141-5)

LOURENCO, KESIA SILVA; DE ASSIS COSTA, OHANA YONARA; CANTARELLA, HEITOR; KURAMAE, EIKO EURYA. Ammonia-oxidizing bacteria and fungal denitrifier diversity are associated with N2O production in tropical soils. SOIL BIOLOGY & BIOCHEMISTRY, v. 166, p. 13-pg., 2022-03-01. (18/20698-6, 13/50365-5, 14/24141-5)

OLIVEIRA, BRUNA G.; LOURENCO, KESIA S.; CARVALHO, JOAO LUIS N.; GONZAGA, LEANDRO C.; TEIXEIRA, MARIA CAROLINA; TAMARA, ANA FLAVIA; SOARES, JOHNNY R.; CANTARELLA, HEITOR. New trends in sugarcane fertilization: Implications for NH3 volatilization, N2O emissions and crop yields. Journal of Environmental Management, v. 342, p. 10-pg., 2023-06-03. (17/02299-4, 19/12557-6, 18/20698-6, 18/20793-9, 17/11523-5)

CARVALHO, JOAO LUIS NUNES; OLIVEIRA, BRUNA GONCALVES; CANTARELLA, HEITOR; CHAGAS, MATHEUS FERREIRA; GONZAGA, LEANDRO CAROLINO; LOURENCO, KESIA SILVA; BORDONAL, RICARDO OLIVEIRA; BONOMI, ANTONIO. Implications of regional N2O-N emission factors on sugarcane ethanol emissions and granted decarbonization certificates. RENEWABLE & SUSTAINABLE ENERGY REVIEWS, v. 149, OCT 2021. (18/20698-6, 18/20793-9, 19/12557-6, 17/02299-4)

LOURENCO, KESIA SILVA; CANTARELLA, HEITOR; SOARES, JOHNNY RODRIGUES; GONZAGA, LEANDRO CAROLINO; DE CAMPOS MENEGALE, PAULA LEITE. DMPP mitigates N2O emissions from nitrogen fertilizer applied with concentrated and standard vinasse. Geoderma, v. 404, DEC 15 2021. (13/50365-5, 18/20698-6, 18/20793-9, 13/12716-0)

LOURENCO, KESIA SILVA; SULEIMAN, AFNAN KHALIL AHMAD; PIJL, AGATA; CANTARELLA, HEITOR; KURAMAE, EIKO EURYA. Dynamics and resilience of soil mycobiome under multiple organic and inorganic pulse disturbances. Science of The Total Environment, v. 733, SEP 1 2020. (13/50365-5, 14/24141-5, 18/20698-6)