Effects and healing time analysis on the use of polyhexamethylene biguanide (PHMB) in dog wounds

• Grant number: 19/19577-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2019
• End date: November 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Bruno Watanabe Minto
• Grantee: João Vitor dos Santos Alves da Silva
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Polyhexamethylene biguanide (PHMB) is a substance with antibacterial properties that has unique and beneficial results in humans. Deepening the study of its effects on companion animals is extremely relevant, potentially promoting better options in the treatment of skin wounds. Thus, this study aims to analyze the therapeutic actions, with emphasis on wound healing time after the use of PHMB as a treatment in dogs. Thirty adult non-castrated dogs of different breeds weighing between 10 and 30 kg who are in good health and do not use medications will be used. The animals will be submitted to elective ovariohysterectomy or orchiectomy procedure and simultaneously to the creation of a circular skin lesion in the abdominal region, with a diameter of 1 cm. They will be divided into 3 experimental groups of 10 animals. The animals of groups 1 and 2 will be submitted to application of dressings filled by PHMB, with changes every 12 and 24 hours, respectively. Group 3 subjects will be treated with topical application of 0.9% saline solution (control group). Macroscopic evaluations of the healing process will occur daily until 10 days in both groups, which will be evaluated according to the presence of edema, exudate, cosmetic appearance and coloration. At the end of the 10 day period, an excisional biopsy of the wound healing area will be performed allowing histopathological, histomorphometric and immunohistochemical evaluation. Statistical analyzes will be performed using the Kruskal-Wallis test for comparison between groups 1, 2 and 3, in relation to the microscopic and macroscopic variables, with subsequent use of the Dunn multiple comparison test in cases in which there is a significant difference between the groups medians. For all tests, p values equal to or less than 0.05 (p <0.05) will be considered significant. For the analysis, the npar1way procedure of SAS (SAS 9.1, SAS Institute, Cary, NC, USA) software and GraphPad Prism, Version 4.00 will be used.