Determination and comparison of Mycoplasma hyopneumoniae shedding capacity in pigs submitted to an innovative oral immunization protocol

• Grant number: 20/08028-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2020
• End date: July 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Luís Guilherme de Oliveira
• Grantee: Geovana Coelho Ferreira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Mycoplasma hyopneumoniae, the main etiological agent of Porcine Enzootic Pneumonia, is a microorganism widely spread in swine farming worldwide. Its prevention is of great interest to the productive system, whereas its colonization in the lung tissue leads to intense productive losses. The commercial vaccines available minimize lung damage and clinical signs; however do not prevent colonization and shedding of the pathogen. Therefore, this study aims to compare the capacity of shedding of M. hyopneumoniae in pigs experimentally infected with this pathogen submitted to different immunizationprotocols, such as (I) immunization with an oral vaccine newly developed by the research group, (II) immunization with broadly used commercial vaccine, and (IIII) in animals which will not receive any immunization protocol. The use of the oral vaccine aims to increase the immune response of the respiratory mucosa by stimulating the intestinal mucosa, and consequently, reducing the shedding of the pathogen. For this purpose, 30 piglets will be divided into three groups (n = 10), with Group 1 (CV) of piglets vaccinated with a single dose commercial vaccine at 24 days of age, Group 2 (OV) of piglets vaccinated with the oral vaccine at 24 days of age, and Group 3 (NC) will constitute the control group. All animals will be challenged at 70 days of age with 5 mL of culture medium containing 106 CCU/mL of M. hyopneumoniae, administered by the tracheal route. Samples of oral fluids will be collected from each pen every three days, while laryngeal and nasal swabs will be collected weekly to monitor the presence and excretion of the agent by qPCR. The normality of the variables will be verified by the Shapiro-Wilkins test. In case of normality, analysis of variance (ANOVA), simple T test and paired T test will be applied for the comparison between groups at different times. If there is no normality, non-parametric tests (Wilcoxon) will be used.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, GEOVANA C.; SANCHES, THAINARA V. C.; MECHLER-DREIBI, MARINA L.; ALMEIDA, HENRIQUE M. S.; STORINO, GABRIEL Y.; SONALIO, KARINA; PETRI, FERNANDO A. M.; MARTINS, TEREZA S.; DA SILVA, LUIS CARLOS CIDES; MONTASSIER, HELIO J.; et al. Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae. Research in Veterinary Science, v. 158, p. 10-pg., 2023-03-31. (20/04707-5, 18/12742-5, 17/17844-8, 19/19710-4, 20/08028-5, 21/11914-0)