The effects of maternal obesity on offspring health focussed on the role of adipose tissue miRNA

• Grant number: 19/22635-4
• Support Opportunities: Scholarships abroad - Research
• Start date: January 04, 2021
• End date: January 03, 2022
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Luciana Pellegrini Pisani
• Grantee: Luciana Pellegrini Pisani
• Host Investigator: Susan Ozanne
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Institution abroad: University of Cambridge, England

Abstract

Maternal obesity during pregnancy and/or lactation is associated with an increased risk of obesity and related metabolic disorders in the offspring throughout life. Molecular mechanisms associated with maternal obesity in the offspring, are still not completely understood. Recently, several groups are studying the effects of dysregulated miRNAs expression on the onset of a variety of metabolic diseases. MiRNAs are short endogenous non-coding RNAs that are able to regulate the cellular protein translation and disturbances in miRNA expression profile have been related to pathological conditions such as obesity and type 2 diabetes. Thus, the aims of the study are to establish the full spectrum of miRNAs programmed in adipose tissue by maternal obesity; determine which miRNAs identified are programmed cell autonomously and expressed in programmed pre-adipocytes in vitro. For this happen, after the animals' treatment will be made small RNA sequencing for miRNA profiling; and isolation of primary preadipocytes and differentiation in-vitro. In summary, with the experimental data from this study, we will be able to provide a better understanding of the mechanisms involved in the programming of adipose tissue microRNA by maternal obesity, as well as present new putative targets for obesity diseases therapy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENNITTI, LAIS VALES; CARPENTER, ASHA A. M.; LOCHE, ELENA; PANTALEAO, LUCAS C.; FERNANDEZ-TWINN, DENISE S.; SCHOONEJANS, JOSCA M.; BLACKMORE, HEATHER L.; ASHMORE, THOMAS J.; PISANI, LUCIANA PELLEGRINI; TADROSS, JOHN A.; et al. ffects of maternal diet-induced obesity on metabolic disorders and age-associated miRNA expression in the liver of male mouse offsprin. International Journal of Obesity, v. 46, n. 2, OCT 2021. (19/22635-4, 14/10683-0, 17/16637-9)

SCHOONEJANS, JOSCA M.; BLACKMORE, HEATHER L.; ASHMORE, THOMAS J.; PANTALEAO, LUCAS C.; PISANI, LUCIANA PELLEGRINI; DEARDEN, LAURA; TADROSS, JOHN A.; AIKEN, CATHERINE E.; FERNANDEZ-TWINN, DENISE S.; OZANNE, SUSAN E.. Sex-specific effects of maternal metformin intervention during glucose-intolerant obese pregnancy on body composition and metabolic health in aged mouse offspring. Diabetologia, v. 65, n. 12, p. 14-pg., 2022-09-16. (19/22635-4)