Effect of local injection of mesenchymal stem cells combined with systemic injections of parathyroid hormone and pentoxifylline on bone repair

• Grant number: 20/03616-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: October 01, 2020
• End date: December 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Adalberto Luiz Rosa
• Grantee: Jaqueline Isadora Reis Ramos
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 17/12622-7 - Cell therapy: potential of mesenchymal stem cells, VEGF-A and BMP-9 to regenerate bone tissue, AP.TEM

Abstract

Bone tissue shows great regeneration capacity when injured by trauma, inflammation or neoplasms. However, in many situations the damage exceeds its regeneration capacity. For these cases, some additional treatments are needed, including pharmacological therapy based on anabolic drugs to bone tissue and cell therapy based on local cell injections. For pharmacological therapy, two clinically available drugs have anabolic effect on bone tissue: Parathyroid Hormone (PTH) and pentoxifylline (PTX), both being able to increase bone formation. For cell therapy, a variety of cells either undifferentiated, such as Mesenchymal Stem Cells (MSCs) or differentiated, such as osteoblasts, may be used. These cells share similar high potential of bone induction when locally injected into defects, although they are not able to bone regeneration. In keeping with this, the aim of this study is to evaluate the effect of combining local injection of bone marrow MSCs with systemic administration of PTH and PTX on bone repair. For this, 5 mm-defects will be made in rat calvaria and to mimic preexistent defects the treatments will begin only after two weeks of defect creation. Firstly, the dose-dependent effect of PTH and PTX on bone repair will be evaluated in order to select the most effective dose of both, then the efficacy of the combination of PTH and PTX will be evaluated. Based on these results the effect of combining local injection of MSCs with systemic injections of PTH, PTX and PTH+PTX on bone repair will be evaluated. Our hypothesis is that the combination of MSCs with PTH+PTX will be able to induce the full regeneration of rat calvaria defects. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RAMOS, Jaqueline Isadora Reis. Effects of local use of mesenchymal stem cells and systemic use of parathormone and pentoxifylline in bone defect repair. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.