Production and evaluation of the use of 2D and 3D lung organoid in host pathogen interaction and analysis of the effect of chitosan nanoparticles

• Grant number: 20/09919-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: December 29, 2020
• End date: December 28, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Carlos Pelleschi Taborda
• Grantee: Suélen Andreia Rossi
• Supervisor: Oscar Zaragoza
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Institut de Salud Carlos III, Majadahonda, Spain
• Associated to the scholarship: 17/25780-0 - Presentation by human dendritic cells of peptides with vaccine capacity and prospection of pan-fungal antigens in paracoccidioidomycosis, BP.PD

Abstract

Organoids are structures formed in vitro that resemble organs in vivo, originated from pluripotent stem cells (PSC) or adult stem cells (ASC). Studies involving interaction of fungal pathogens on organoids are scarce. Therefore, there is great interest in deepening the understanding of the use of this technology to produce information from the infection, development and treatment of fungal infections. Gp43 is a 43kDa glycoprotein from Paracoccidioides brasiliensis and the P10 peptide, originated from a specific portion of the gp43, has been investigated as an experimental vaccine. Studies using encapsulated peptide P10 nanoparticles have shown that the use of nanocarriers assists in the effect of antifungal therapy against paracoccidioidomycosis. The main objective of this project is to use human 2D-lung organoid culture to elucidate the dynamics of fungal pathogen interaction and to evaluate the response obtained after contact of nanoparticles with encapsulated P10. Besides, standardization of the infection model in human 3D-lung organoid will be carried out, as well as the production and maintenance of the 3D culture cell. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, HAROLDO CESAR; ROSSI, SUELEN ANDREIA; GARCIA-BARBAZAN, IRENE; ZARAGOZA, OSCAR; TREVIJANO-CONTADOR, NURIA. Cell Wall Integrity Pathway Involved in Morphogenesis, Virulence and Antifungal Susceptibility in Cryptococcus neoformans. JOURNAL OF FUNGI, v. 7, n. 10, OCT 2021. (20/09919-0)