Development of a carbon flux control system in synthetic metabolic pathways in yeast

• Grant number: 20/06165-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2021
• End date: April 30, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Danielle Biscaro Pedrolli
• Grantee: Maria Cecília de Souza Lelis
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Isoprenoids are one of the most abundant classes of natural products in the plant kingdom. They have applications in miscellaneous areas, such as pharmaceutical, agriculture and biomaterials. In this manner, the heterologous expression in microorganisms emerges as the quickest option to fulfill the commercial demand. However, the metabolic route of terpenoids competes for cell supply with cellular machinery. The strain Saccharomyces cerevisiae ILHA LIMI4A was constructed longing to increase the terpenes production, yet the cell suffers from the accumulation of isopentane pyrophosphate (IPP), one of the precursors in the isoprenoid pathway, which is toxic to the cell in high concentrations. Through the reconstruction of IDI1, ERG20w and CILS genes, this project aims to enhance the carbon flux through the monoterpenes biosynthetic route and to reduce the cytoplasmic disponibility of IPP, seeking to direct the carbon flux for limonene production. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LELIS, Maria Cecília de Souza. Desenvolvimento de sistema de controle de fluxo de carbono em vias metabólicas sintéticas de levedura. 2022. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.