Crosstalk between microbiota and histone modifications in the healthy gut

• Grant number: 21/00398-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2021
• End date: February 28, 2026
• Field of knowledge: Biological Sciences - Immunology - Immunogenetics
• Principal Investigator: Patrick Daniel Varga-Weisz
• Grantee: Dieggo Rodrigues de Paula
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 19/16113-5 - Chromatin dynamics in host-microbiome crosstalk in health and disease, AP.SPEC
• Associated scholarship(s): 23/10157-6 - Evaluation of histone post-translational acylations landscape caused by short-chain fatty acids in the gut, BE.EP.DD

Abstract

Histone modifications are a key component of the gene regulatory machinery. However, we know little what role they play in the important microbiota-host crosstalk in the colon epithelium. We will build on work from the Varga-Weisz and Vinolo laboratories that identified histone crotonylation as a modification that is dependent on the microbiota in the colon. We will use the mouse system to address three complementary questions: (I) how is histone crotonylation linked to gene expression genome-wide; (II) how are histone crotonylation and other modifications affected globally by the microbiota, using mass spectrometry and (III) how are changes in histone crotonylation linked to changes in chromatin structure. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NIRELLO, VINICIUS D.; DE PAULA, DIEGGO RODRIGUES; ARAOJO, NATHALIA V. P.; VARGA-WEISZ, PATRICK D.. Does chromatin function as a metabolite reservoir?. TRENDS IN BIOCHEMICAL SCIENCES, v. 47, n. 9, p. 4-pg., 2022-08-11. (21/05269-4, 19/16113-5, 21/00398-0, 21/00393-9)

VAI, ALESSANDRO; NOBERINI, ROBERTA; GHIRARDI, CHIARA; DE PAULA, DIEGGO RODRIGUES; CARMINATI, MICHELE; PALLAVI, RANI; ARAUJO, NATHALIA; VARGA-WEISZ, PATRICK; BONALDI, TIZIANA. Improved Mass Spectrometry-Based Methods Reveal Abundant Propionylation and Tissue-Specific Histone Propionylation Profiles. MOLECULAR & CELLULAR PROTEOMICS, v. 23, n. 7, p. 14-pg., 2024-07-01. (19/16113-5, 21/00398-0, 21/05269-4, 23/10157-6)