Comparison of HepG2 cells culture in monolayer (2D) and three-dimensional (3D) at hepatotoxicity evaluation induced by decamethylcyclopentasiloxane (D5) and triclopyr

• Grant number: 20/11128-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: October 01, 2021
• End date: April 22, 2026
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Lilian Cristina Pereira
• Grantee: Natália Akemi Kohori
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Humans are exposed to a multitude of day-to-day chemicals: pesticides, pharmaceuticals and industrial chemicals, among other substances. To ensure adequate protection of human health and the environment, it is essential that the potential of these compounds to induce toxicity is adequately verified. Experimental studies have the capacity to predict the toxicity of these emerging contaminants and are valuable aids for understanding biological mechanisms potentially involved in the risk assessment to human health. Currently, the use of so-called alternative methods to animal use for assessing the toxicity of chemicals is an international trend in response to the 3R's policy that restricts the use of animals in research. Among the new alternative methodologies applied to toxicology include cell cultures in monolayers (2D), three-dimensional (3D) and 3D bioprinting. This methodology can contribute to elucidate possible mechanisms of toxicity of emerging environmental contaminants. And building a framework to Adverse Outcome Pathways (AOPs) for its. Then elucidated presence of the mitochondrial dysfunctions, autophagy/mitophagy, degeneration and cell death. This project, which puts us in line with the worldwide trend of using alternative methods to assess the risk assessment of chemical compounds and use of AOPs, aims to evaluate the toxicities of two environmental contaminants, the herbicide triclopyr and the product for personal use in cosmetics, decamethylcyclopentasiloxane (D5). The indicated experimental system - hepatic (HepG2) cell culture in monolayer (2D), 3D and 3D bioprinting may provide scientific support for the implementation of coherent and effective strategies to control these environmental contaminants. (AU)