In vitro and in vivo study of nano-mil-100 (fe) containing piperin coated with macrophagus membrane vesicules for the treatment of breast cancer

• Grant number: 21/07281-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 07, 2022
• End date: October 20, 2022
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Marlus Chorilli
• Grantee: Christian Rafael Quijia Quezada
• Supervisor: Alberto Ocaña Fernandez
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Universidad de Castilla-La Mancha, Albacete (UCLM), Spain
• Associated to the scholarship: 18/21119-0 - Evaluation of the potential of piperine encapsulated in metal-organic frameworks coated with macrophage membranes in the treatment of Breast Cancer, BP.DR

Abstract

Breast cancer is the second most common type of cancer and is the leading cause of death among women worldwide, with an estimated 13 million deaths in 2030. Although there are many treatments available, many types are used today chemotherapy. Among the compounds with chemotherapeutic potential is piperine (PIP), it has anticancer and antitumor activity against breast cancer both in in vitro and in vivo studies. However, the introduction of this compound in preclinical studies has been limited, due to the high concentration requirement and its hydrophobic nature, causing problems related to bioavailability and immunotoxicity. Encapsulation of PIP in nano-MIL-100 (Fe) would facilitate drug delivery and could improve biopharmaceutical properties. In addition, macrophage membranes (MM), would help to be a camouflage system against the body's immune system. In order to improve its solubility and reduce the non-specific toxicity of PIP, we generated nano-MIL-100 (Fe) containing PIP and coated with macrophage membrane vesicles (MM @ PIP @ nano-MIL-100 (Fe)). Therefore, in the present study, we will evaluate the antitumor efficacy of MM @ PIP @ nano-MIL-100 (Fe) both in vitro and in a murine model of breast cancer. (AU)