Development of an analytical methodology to evaluate the modulation role of HDL in activated macrophages

• Grant number: 21/12886-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: July 31, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Graziella Eliza Ronsein
• Grantee: Francielle Aguiar Gomes
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/00696-3 - Proteomic as a tool to understand HDL function and composition, AP.JP

Abstract

Among the plasma lipoproteins, high-density lipoprotein(HDL) is responsible for removing excess cholesterol from macrophages in the artery walls in a process called reverse cholesterol transport. However, other important functions have been attributed to this lipoprotein. For instance, HDL has been shown to modulate the inflammatory response of activated macrophages. In this work, we aim to evaluate the influence of HDL in the inflammatory response of macrophage challenged with LPS and/or excess cholesterol. To do this, we will use macrophages in cell culture and techniques of proteomics to identify the proteins released by macrophages exposed to LPS and/or loaded with cholesterol. In addition, we will evaluate the influence of pretreatment with HDL in the release of these proteins.