Biocompatibility evaluation and scanning electron microscopy analysis of different calcium silicate endodontic cements in contact with connective tissue

• Grant number: 21/07733-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: July 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marina Angélica Marciano da Silva
• Grantee: Juliana Minto Boldieri
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Biocompatibility is one of the most important properties that endodontic cements must have, as they will be in close contact with periapical tissues. These calcium silicate-based repairing and filling materials are presented in ready-to-use or powder/liquid formulas. The aim of this study is to evaluate the in vivo response of biocompatibility, chemical and surface analysis in SEM/EDS of repair cements: Gray MTA Plus (Avalon Biomed Inc., Bradenton, USA), white MTA (Angelus, Londrina, Brazil) and ENDOCEM MTA (Maruchi, Wonju-si, Republic of Korea); and obturators: CeraSeal (Meta Biomed, Heungdeok, Republic of Korea), AH Plus Jet (Dentsply, Konstanz, Germany) and GuttaFlow bioseal (Roeko Coltene, Langenau, Germany). The cements (n=8) will be inserted inside polyethylene tubes and implanted in the subcutaneous connective tissue of rats, 4 tubes per animal: 2 on the right side for histological analysis (one filled with endodontic cement and the other empty characterizing the control); 2 tubes on the left side (filled with the material, one for histological analysis and the other for study in SEM/EDS). Euthanasia will be carried out in periods of 7 and 30 days. Histological analysis will be performed on 5 ¼m thick sections for staining with hematoxylin-eosin and 10 ¼m sections for staining with the von Kossa technique, to observe mineralization and the presence of inflammatory infiltrate. The results will be submitted to the Kruskal-Wallis test (p < 0.05) to analyze the results in scores. (AU)