Interaction of PRC2/EZH2 complex and lncRNAs in thyroid Cancer

• Grant number: 21/14494-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2022
• End date: December 31, 2022
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Cesar Seigi Fuziwara
• Grantee: Amanda Luvisotto Gomes Leite Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 19/17282-5 - Transcriptional and post-transcriptional control in aggressive cancer and metastasis, AP.JP

Abstract

The increase in PRC2/EZH2 Polycom complex activity in aggressive cancer induces gene silencing through histone modifications and chromatin accessibility. However, the mechanism underlying the targeting of PRC2/EZH2 to specific regions of the genome is under investigation. In this context, long noncoding RNAs emerge as potential mediators as they can recruit and Interact with PRC2/EZH2. Classically, it is known that this interaction occurs in the X chromossome inactivation via lncRNA XIST, for example. Despite the description of lncRNA deregulation in different types of thyroid cancer, its interaction with PRC2/EZH2 is still elusive. In this project, we aim to investigate the interaction of lncRNAs with the PRC2/EZH2 complex and its effects in aggressive thyroid cancer using anaplastic thyroid cancer cell line and RNA immunoprecipitation. We expect to identify new lncRNA that Interacts with PRC2/EZH2 and contributes to thyroid cancer oncogenesis.(AU)