Light-based regulation of the miRNA156/SPL9 module in the early hypocotyl elongation

• Grant number: 21/14640-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: April 03, 2022
• End date: April 02, 2023
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Fabio Tebaldi Silveira Nogueira
• Grantee: Flaviani Gabriela Pierdoná
• Supervisor: Vincent Ullas Pedmale
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: Cold Spring Harbor Laboratory (CSHL), United States
• Associated to the scholarship: 20/12940-1 - Study of the interaction between the microRNA156/SPL module and two hormones (brassinosteroid and gibberellin) during early hypocotyl growth, BP.DR

Abstract

The light perception in seedlings modulates the post-embryonic development, integrating phytohormone and other endogenous responses with environmental cues. A proper hypocotyl elongation at the first hours/days of development is critical for plant establishment and photosynthetic success. The precise coordination of hypocotyl elongation is under parallel and redundant regulatory pathways. Gene expression, at specific cells, modulated by photoreceptors and phytohormones, is the main regulatory mechanism at this phase. However, the integrative pathways controlling hypocotyl development are not completely elucidated. Our previous results showed that themiRNA156/SPL9 module is associated with hypocotyl elongation control. We hypothesized that this module acts as a regulatory hub in gibberellin (GA), brassinosteroid (BR), and light crosstalk. We already generated several data indicating that SPL9 expression is modulated by GA and BR, and perhaps by light during hypocotyl elongation. The BEPE project proposes to evaluate in greater detail th ehypocotyl phenotype and transcriptome alterations in Arabidopsis miR156/SPL9mutants and transgenic lines under distinct light regimes. The hypocotyl length from seedlings growing in high red/far-red, and low blue/UV-A light will show whether the phenotype observed in SPL9 gain or loss of function is dependent on the light quality. Moreover, transcriptome analyses of specific cells in the hypocotyl expansion zone under distinct light regimes will provide important insights into the SPL9 function as an activator and/or repressor of light responses. The use of SPL9-GFP transgenic plants will allow the discovery of direct targets by ChIP-qPCR. Finally, the combination of RNA-seq and ChIP-qPCR will allow the discovery of SPL9 direct targets, which maybe modulated by BR, GA, and light in the hypocotyl. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIERDONA, FLAVIANI G.; CARBAJAL, YAJAHAIRA; VICENTE, MATEUS H.; FERIGOLO, LETICIA F.; NOGUEIRA, FABIO T. S.. Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol. BIO-PROTOCOL, v. 12, n. 24, p. 10-pg., 2022-12-20. (18/17441-3, 20/12940-1, 19/20157-8, 21/14640-8)