USE OF DISSOLVING MICRONEEDLES CONTAINING AMINOLEVULINIC ACID FOR PHOTODYNAMIC THERAPY

• Grant number: 22/10860-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2022
• End date: March 14, 2025
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Michelle Barreto Requena
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID
• Associated scholarship(s): 23/04209-3 - Dissolving microneedles for nonmelanoma skin cancer and melanoma treatments with PDT and SDT: basic studies and pre-clinical experiments, BE.EP.PD

Abstract

Topical photodynamic therapy (PDT) is a non-melanoma skin cancer treatment technique. Our research group is a pioneer in the country in the field, exploring from basic science to clinical studies. Over the years, we have carried out projects to develop protocols to improve cure rates and patient comfort. However, there are still some limitations that hinder medical acceptance of the technique in clinical practice, especially the limited penetration of both cream and light. In addition, the treatment time is considered long, as it depends on the incubation time for photosensitizer (PS) accumulation into the lesion. Our PDT optimization studies have explored intradermal drug delivery techniques to address these limitations, and the results demonstrate it is possible to achieve greater depths and homogeneity in PS distribution. In this context, dissolving microneedles are an intradermal delivery system with the potential to increase active principle penetration and decrease incubation time, and also improve light-tissue coupling. This project aims to microneedles development, from the manufacture and characterization processes through stability evaluation, development of an ozone decontamination protocol, animal tumor model studies, up to an unprecedented pilot clinical study in patients with basal cell carcinoma. In parallel, experiments will be conducted to evaluate microneedles as light guides, aiming to increase light penetration into the lesion. This study shall provide an understanding of fundamental aspects of a technology not yet explored in PDT, obtaining a model with better performance and cost-effectiveness for clinical application.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARRERA-PATINO, C. P.; DE OLIVEIRA, LUCAS ORLANDI; MATTOS, VICENTE SILVA; STRINGASCI, MIRIAN DENISE; REQUENA, MICHELLE BARRETO; BAGNATO, VANDERLEI SALVADOR. Skin microvasculature determined using OCT angiography: first evidence of its relation with aging. LASER PHYSICS LETTERS, v. 20, n. 2, p. 6-pg., 2023-02-01. (13/07276-1, 14/50857-8, 22/10860-6)

SALVIO, ANA GABRIELA; REQUENA, MICHELLE BARRETO; STRINGASCI, MIRIAN DENISE; FREGOLENTI, BIANCA A.; MEDERO, MAIRA M. C.; SILVA, ROSILENE G. S.; BAGNATO, VANDERLEI SALVADOR. Photodynamic therapy for nodular basal cell carcinoma up to 5mm located on high-risk area: Effectiveness and long-term follow-up results. Photodiagnosis and Photodynamic Therapy, v. 45, p. 3-pg., 2024-02-27. (13/07276-1, 22/10860-6, 14/50857-8)

GARCIA, MARLON RODRIGUES; SANCHEZ, VICTOR; FORTUNATO, THEREZA CURY; REQUENA, MICHELLE BARRETO; GRECCO, CLOVIS; VOLLET-FILHO, JOSE DIRCEU; PIRES, LAYLA; MORIYAMA, LILIAN TAN; PRATAVIEIRA, SEBASTIAO. In Vivo and In Silico Study of Photodynamic Necrosis Volume in Rat Liver. PHOTONICS, v. 9, n. 12, p. 12-pg., 2022-12-01. (14/50857-8, 22/10860-6, 13/07276-1)

PONCE AYALA, ERIKA TONETH; REQUENA, MICHELLE BARRETO; BAGNATO, VANDERLEI SALVADOR; PRATAVICIRA, SEBASTIAO. Quantification of Protoporphyrin IX in Murine Pigmented Melanoma Induced by Systemic and Topical ALA Administration Protocols. OPTICAL METHODS FOR TUMOR TREATMENT AND DETECTION: MECHANISMS AND TECHNIQUES IN PHOTODYNAMIC THERAPY XXXII, v. 12823, p. 8-pg., 2024-01-01. (13/07276-1, 22/10860-6, 14/50857-8, 23/14868-4)

SALVIO, ANA GABRIELA; STRINGASCI, MIRIAN; REQUENA, MICHELLE B.; FREGOLENTI, BIANCA A.; MEDEIRO, MAIRA MONIQUE DA COSTA; SANTOS, ROSILENE GUEDES; BAGNATO, VANDERLEI SALVADOR. Long-term follow-up results of a pilot study for nodular basal cell carcinoma with PDT using partial home treatment protocol. Photodiagnosis and Photodynamic Therapy, v. 45, p. 3-pg., 2024-02-27. (13/07276-1, 22/10860-6, 14/50857-8, 23/04209-3)