Functional analyses of human FMR1 circular transcripts

• Grant number: 22/09710-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2022
• End date: June 30, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Luciana Amaral Haddad
• Grantee: Daniel Key Takemoto
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Fragile X syndrome is due to loss of function of the FMR1 gene, which encodes FMRP, a protein related to neurodevelopment in the central nervous system. Changes in FMRP expression may be deleterious to neurogenesis and synaptic plasticity. Alternative splicing may affect in different ways the FMR1 transcript segment between exon 12 and exon 15. Here we aim to assess the effects of the overexpression of two FMR1 alternative transcripts on the stability of different linear mRNAs, including the FMR1 mRNA itself.