| Grant number: | 22/04349-7 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | November 01, 2022 |
| End date: | October 31, 2025 |
| Field of knowledge: | Health Sciences - Collective Health - Public Health |
| Principal Investigator: | Jancarlo Ferreira Gomes |
| Grantee: | Felipe Augusto Soares |
| Host Institution: | Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated research grant: | 14/12236-1 - AnImaLS: Annotation of Images in Large Scale: what can machines and specialists learn from interaction?, AP.TEM |
Abstract To advance in the intestinal parasite diagnosis in humans, through the Ova and Parasites examination (O&P), it is necessary the scientific development and technological alternatives that can reduce the manual steps of this exam. Thus, increasing human accuracy when interpreting results, while reducing the excessive use of chemical reagents harmful to humans, parasitic structures, and the environment. Dissolved Air Flotation (DAF) has been proved to be a new technical principle with efficient intestinal parasites recovery and removal of fecal impurities (organic debris). The use of this technique would benefit laboratory practices with perspective automation of fecal processing. Therefore, this research aims to develop and validate, with an intra-laboratory setting, the integration of a new mechanized DAF equipment together with an Automated Diagnosis of Intestinal Parasites (DAPI) system. In this study, the integration of these devices is called FADDAPI/G1. In the laboratory development stage, 300 positive fecal samples for intestinal parasites will be used from a volunteer population of the Municipal Health Laboratory (MHL) in Campinas- SP. These samples will be used in standardization trials with emphasis on DAF, to measure the parasite's recovery contained in the floated and non-floated contents. For this evaluation, the following parameters will be tested: (1) investigation of different surfactants and cationic polymers; adjustment of optimal concentrations of chemical reagents, (2) adaptation of the fecal suspension volume in the flotation tube, and (3) adequacy of these parameters in the smears fecal preparation on microscopy slides for endoparasites detection by FADDAPI/G1 system. The intra-laboratory validation of FADDAPI/G1 will be performed by comparing it with the manual parasitological technique of the TF-Test modified and the DAPI system unified with the TF-Test modified. It will be used 400 fecal samples randomly collected taking into account the comparison statistical metrics, such as sensitivity, specificity, and Kappa agreement. The results of this study should provide an unprecedented opportunity for the development of an integrated system for mechanized fecal samples processing, automated detection, and identification by image analysis of intestinal parasites. | |
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