Effect of mTOR pathway stimulation during embryonic culture in the absence or presence of glucose on trophectoderm differentiation in embryos cattle.

• Grant number: 22/02748-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2022
• End date: May 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Demarchi Goissis
• Grantee: Francieli Perroni Berling
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/09576-3 - Studies on cell differentiation mechanisms during early bovine embryo development, AP.JP

Abstract

In vitro embryo production (IVP) allows an accelerated genetic improvement of the herd in a short period. However, the pregnancy rates of IVP embryos are still lower than those produced in vivo and may be related to initial cell differentiation. The first cell differentiation event in the embryo determines the inner cell mass and trophectoderm (TE). In mice, the involved biological mechanisms are well characterized and include theinvolvement of glucose metabolism and the Hippo signaling pathway. The mTOR pathway integrates glucose metabolism with the Hippo signaling pathway and can be stimulated by the S1P lipid, which also influences the Hippo pathway. Incattle, the Hippo pathway seems to have a relevant role in this first differentiation, however other aspects appear different. Therefore, we hypothesize that S1P induces differentiation into TE via mTOR independently of glucose in bovine embryos. To test this, we will perform treatments on embryos produced in vitro in the absence or presence of glucose and treatment or not with S1P. The effects will be verified by q-RT-PCR and immunofluorescence, aiming at the observation ofgenes and proteins related to TE differentiation. It is expected that the treatment of S1P in bovine embryos increases the proportion of TE cells and positively modulates the expression of TE-related genes, regardless of the presence of glucose.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERLING, Francieli Perroni. Effect of Sphingosine-1-phosphate on trophectoderm differentiation in bovine embryos. 2024. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.