Isolation and characterization of extracellular vesicles and evaluation of the expression of cannabinoid receptors CB1 and CB2 in multipotent mesenquimal stromal cells

• Grant number: 22/11569-3
• Support Opportunities: Scholarships in Brazil - Program to Stimulate Scientific Vocations
• Start date: January 04, 2023
• End date: February 23, 2023
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Rosalia Mendez-Otero
• Grantee: Luís Felipe Arantes Moya
• Host Institution: Instituto de Biofísica Carlos Chagas Filho (IBCCF). Universidade Federal do Rio de Janeiro (UFRJ). Ministério da Educação (Brasil)

Abstract

The Endocannabinoid System constitutes a complex set of pathways signaling whose functions range from presynaptic neuronal inhibition to immunomodulation. The endogenous messengers of the system are lipids synthesized on demand (anandamide and 2-arachidonoyl glycerol) that interact with G protein-coupled receptors (CB1 and CB2). CB1 receptors arefound primarily in the central nervous system (CNS) and CB2 in cells peripheral and immune. The affinity of exogenous substances (phytocannabinoids orsynthetic agonists) with these receptors makes it possible to administer these substances for therapeutic purposes of both CNS and immune disorders. At stem cells are able to self-renew and give rise to other cell types through a process known as asymmetric mitosis obtaining and characterization of cell populations from adult individuals as stem cells mesenchymal cells (MSC) is of great applicability in regenerative medicine, through of cell therapy. MSCs can be understood as "living stocks" of substances that, under appropriate conditions, secrete bioactive molecules into extracellular vesicles or in the form of soluble factors and interact with other cells in order to create a regenerating microenvironment. Stimulation of MSC by Cannabinoid receptor agonists may induce anti-inflammatory activity in this cells, with possible future therapeutic applications. This work aims explore the hypothesis that MSCs from different equine tissues express CB1 and CB2 cannabinoid receptors. (AU)