PERFORMANCE AND PERCEPTUAL PARAMETERS ANALYSIS AND ITS RELATIONSHIP WITH INDIRECT MARKER OF MUSCLE DAMAGE INDUCED BY ECCENTRIC EXERCISE AFTER ISCHEMIC PRECONDITIONING: RANDOMIZED PLACEBO CONTROLLED TRIAL

• Grant number: 22/12518-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2023
• End date: December 31, 2023
• Field of knowledge: Biological Sciences - Physiology - Physiology of Effort
• Principal Investigator: Franciele Marques Vanderlei
• Grantee: Renan Alves Matsumoto
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

Introduction: eccentric exercise (EE) has been widely used to improve physical fitness, due to its mechanical advantage and lower metabolic demand. However, its performance can induce muscle damage, stimulating structural changes and reduced muscle function, perceptual changes such as subjective pain sensation and perception of recovery and especially in indirect markers of muscle damage such as creatine kinase (CK). Thus, ischemic preconditioning (IPC) can be seen as an adjunct in reducing the damage caused by EE, in which it can minimize the deleterious effects of ischemia-reperfusion, and have repercussions on better perceptual and performance responses, thus accelerating the process. post-exercise recovery. Objectives: to analyze the behavior of the perceptual responses (pain by the visual analogue scale [VAS], subjective perception of effort by the LIKERT scale and pain threshold by the pressure algometer) and performance (maximum isometric voluntary contraction [MVIC]) after the application of the PCI with different occlusion pressures and correlate them with an indirect marker of muscle damage (CK). Methods: a randomized placebo-controlled clinical trial will be performed with 80 healthy men aged 18 to 35 years who will be randomly divided into four groups: PCI using total occlusion pressure (POT), PCI with 40% more than POT, placebo (10 mmHg) and control. The PCI and placebo protocol will consist of four cycles of ischemia and reperfusion of five minutes each, while the control will remain at rest in the supine position. All groups will perform the initial evaluations, an EE protocol, evaluations immediately after the end of the EE, 24, 48, 72 and 96 hours after the exercise, being sequentially evaluated the CK through the Reflotron Plus System, EVA and LIKERT by demonstration of the scales, pain threshold by pressure algometer and CVIM by isokinetic dynamometer. The descriptive statistical method and analysis of variance will be used for the repeated measures model and linear regression. The significance level will be p<0.05.