USE OF "OMIC" (PROTEOMIC AND METABOLOMIC) APPROACHES TO ELUCIDATE THE SYNERGISM OR ANTAGONISM IN THE TOXICITY OF AMETRIN AND CLOMAZONE IN ZEBRAFISH

• Grant number: 22/14467-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2023
• End date: February 29, 2024
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Fábio Erminio Mingatto
• Grantee: Camila Araújo Miranda
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil

Abstract

Environmental contamination by agricultural residues is a worldwide reality, the use to maximize production has been increasingly frequent by farmers due to the high efficiency in pest control and the possibility of producing more in the same area. The constant use associated with the incorrect application makes this a public health problem considering that these residues accumulate through biological chains many times, putting human and animal health at risk. Every year in Brazil different pesticides are legalized for the control of various agricultural and veterinary pests, it is known that not all people have the technical knowledge to correctly choose which compound to use and mistakenly end up applying different products in a short time to reduce the damage caused, the interaction of these components can potentiate the toxic effects on non-target organisms and affect directly and indirectly the environment. In plantations, the simultaneous application of two or more compounds that act in different ways to control weeds is increasingly common. After irrigation or rain, these compounds undergo a leaching process and can reach the tributaries. In the literature, data regarding contamination and environmental toxicity caused by ametrine, clomazone and commercial product containing the two herbicides in the formulation were not fully described and therefore further studies are necessary. To clarify some aspects, we will use the zebrafish (Danio rerio) as a study model to evaluate the changes caused after exposure to different concentrations of the substances mentioned above. Considering that this is a model widely accepted by the scientific community, the results are expected to provide important information about the toxic effects caused in animals.