Avaliation of post-incisional analgesia with meloxicam, morphine and crotalphine in Wistar Rats.

• Grant number: 23/06838-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2023
• End date: July 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Annelise Carla Camplesi dos Santos
• Grantee: Julia Costa Baroni
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Post-incisional pain is a very common type of acute pain that is still under-treated. The experience of this type of pain leads to increased morbidity, mortality and recovery time, therefore, effective analgesic protocols are of major importance. The main drugs used are non-steroidal anti-inflammatories and opioids, however, due to the high potential for adverse effects, the search for alternative therapies is growing. Given this importance, the synthetic peptide crotalfin has shown promise in previous studies in models of acute pain, chronic pain and neuropathic pain, inducing long-term analgesia in all models and so far showed no adverse effects related to its use. The aim of this study is to compare the analgesia from crotalfin with analgesics frequently used in clinical settings as morphine and meloxicam and for this, 30 Wistar rats will be used, with an average weight of 300g, which will undergo the surgical procedure described by the Model of Incisional Pain in the Paw, and then will be divided into 3 groups that will receive the respective analgesic drugs: morphine (representative of opioids), meloxicam (representative of non-steroidal anti-inflammatory drugs) and crotalphine. Pain will be evaluated using the Von Frey Method and the Open Field Method, before the surgical procedure, immediately after the application of analgesic procedures (2 hours after the procedure), and then 4, 6, 12, 24, 48, 72, 96 and 120 hours after the incisional procedure. Thus, after analysis and interpretation of the results obtained in the conditions of the experiment, the comparison of the analgesia of the peptide crotalfin will show if this substance can be another alternative to be used clinically in analgesic protocols for post-incisional pain.