ANATOMICAL AND RADIOGRAPHIC STUDY OF THE TONGUE AND PALATINE TONSILS IN BRACHYCEPHAL AND MESOCEPHALLIC DOGS

• Grant number: 23/06613-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2023
• End date: July 31, 2024
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Fabrício Singaretti de Oliveira
• Grantee: Karen Wassilewsky Caetano
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Fixation and conservation methods are used to preserve cadavers for the study of anatomy for a longer period of time, without the proliferation of microorganisms. Several studies have demonstrated the effectiveness of glycerin ethyl alcohol for fixation and curing salts for the conservation of cadavers, keeping the morphological characteristics of cadavers very close to fresh animals. The objective of this research is to carry out an anatomical and radiographic study of the tongue and palatine tonsils of brachycephalic and mesocephalic dogs, in order to assist in the treatment of these animals in the veterinary clinical-surgical routine, in addition to evaluating the applicability of the anatomical technique used for the evaluation of the irrigation of the tongue and tonsil using radiography in cadavers chemically prepared with glycerin ethyl alcohol, curing salts and latex with barium sulfate for up to 120 days of refrigeration. Fifteen cadavers of brachycephalic dogs and 15 of mesocephalic dogs will be used, preserved with 120 mL/kg of curing salt, 120 mL/kg of alcohol with 5% glycerin and injected with latex mixed with barium sulfate. They will be radiographed in order to verify the radiographic anatomy of the palatine tonsil and tongue arterial vascularization. This research will contribute to a better anatomical and radiographic knowledge of brachycephalic dogs, referring to the vascularization of the tongue and palatine tonsil, which is essential for the success of the clinical and surgical approach. In addition, the proposed chemical conservation should reduce the number of discarded carcasses, will not cause soil and groundwater contamination, reducing the impact caused on the environment, as it will be possible to use the same cadaver for different approaches (anatomy and radiographic) by up to 4 months, just kept under refrigeration, being possible to handle it several times and without risk to the researchers involved.