CHITOSAN-CALCIUM SCAFFOLDS FUNCTIONALIZED WITH MAGNESIUM OXIDE FOR DENTIN REGENERATION

• Grant number: 23/06685-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: August 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Diana Gabriela Soares dos Passos
• Grantee: Beatriz Esteves Pelicer
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Currently, the development of biomaterials into Regenerative Dentistry has been into the spotlight, being studied in association with tissue engineering concepts. Novel technologies have been developed to aid dentin regeneration, by creating from novel biomaterials a favorable environment for dental pulp cells proliferate and differentiate, leading to pulp-dentin complex regeneration. This research project has the aim to develop macro-porous chitosan calcium hydroxide scaffolds, functionalized with magnesium oxide for dentin regeneration. Morphological (SEM/DES) and biological characterization will be performed with dental pulp cells seeded in direct contact with materials. The following analyses will be performed: cell viability (Live/Dead), cell metabolism (Alamar Blue), and mineralized matrix deposition (Alizarin Red). Numerical data will be subjected to statistical analysis.