DEVELOPMENT AND ANALYSIS OF A TITANIUM SURFACE MODIFICATION USING COLLAGEN AND NARINGENIN: EFFECT ON OSTEOBLASTS IN IN VITRO MODEL

• Grant number: 23/05125-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: August 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Fernanda Gonçalves Basso
• Grantee: Leonardo Victoriano Inácio
• Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil

Abstract

The success on the installation of osseointegrated implants depends on the formation of an effective Anchorage of these components to the bone tissue as also on peri-implant mucous biological sealing. These events are related to the adhesion of bone and mucous cells to implants surface as also to the response of these cells following an inflammatory stimulus. The incidence of failures of osseointegrated implants in the oral cavity may be related to an increased synthesis of matrix metalloproteinases (MMPs). Besides the physiological activity of these enzymes as collagenases during tissue remodeling the super-expression of these proteins may lead to extended extracellular matrix degradation and delay of repair process. Considering these events, different therapeutic strategies may facilitate the peri-implant repair, accelerating the resolution of the inflammatory phase have been investigated. Among them, the use of bioflavonoids have demonstrated effective on the response of osteoblasts and gingival fibroblasts, modulating the proliferation and expression of inflammatory mediators. Therefore, this investigation will develop a modification of a titanium surface using collagen associated or not with naringenin (citrus bioflavonoid) and evaluate the effect of this functionalization on the adhesion and metabolism of osteoblast using an in vitro model. Data will be statistically analyzed considering distribution and homoscedasticity.