Evaluation of different production and storage techniques for blood components eye drops as a proposal for treating ocular surface diseases

• Grant number: 23/07038-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2023
• End date: April 30, 2027
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Mônica de Cássia Alves
• Grantee: Mariane Aparecida Risso
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated research grant: 21/04045-5 - Evaluation of new techniques of cellular therapy to ocular surface diseases, AP.JP2

Abstract

Ocular surface disease (OSD) is a multifactorial condition of the ocular surface with a great impact on quality of life and epidemiological importance. The use of blood products and components has gained more space for their treatment. Autologous serum (AS) is now widely used with proven efficacy for treating ocular surface disease. Platelet-rich plasma (PRP) has also been shown to be effective, with promising results, as has the use of plasma rich in growth factor (PRGF). The main objective of this project is to systematize the preparation of AS, PRP and PRGF, testing their frozen, cryopreserved and lyophilized storage, to verify the impact of different preparation and storage techniques on the dosages of growth factors (EGF, TGF-²1, VEGF and PDGF), pH and its biological potential in ocular tissue cell culture. Scientific research on new therapeutic modalities related to the systematization of the production of blood component eye drops is a partnership between the Department of Ophthalmology at the State University of Campinas - Unicamp, the BTTC (Bank of Tissues and Cellular Therapy) and LNBio (National Laboratory of Biosciences) of CNPEM (National Center for Research in Energy and Materials). It represents an excellent opportunity for research and technological and scientific advances in DSO, with proposals for therapeutic options and conservation methods appropriate for the production and distribution of these products on a large scale.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RISSO, MARIANE APARECIDA; BARQUILHA, CAROLINE NASCIMENTO; NUNES HOLZHAUSEN, HELGA CAPUTO; GROSS, LUISA GRAVE; DE AZEVEDO, RAFAEL JUNIOR; HARB, SAMARAH VARGAS; LEME, KRISSIA CAROLINE; LUZO, ANGELA; MIGLIORINI FIGUEIRA, ANA CAROLINA; ALVES, MONICA. Comparative Analysis of Platelet -Rich Plasma and Platelet -Rich Fibrin Gels as Advanced Therapy Medicinal Products for Ophthalmic Applications. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v. 65, n. 7, p. 4-pg., 2024-06-01. (21/04045-5, 23/07038-5)