In vitro probiotics characterization and evaluation of the inhibitory potential of the growth of pathogenic bacteria causing urinary tract infection in dogs by lactic acid-producing bacteria from the genital microbiota of healthy animals

• Grant number: 23/08877-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2023
• End date: October 31, 2026
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Priscylla Tatiana Chalfun Guimaraes-Okamoto
• Grantee: Reiner Silveira de Moraes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Urinary tract infections (UTI's) have an important an important morbidity rate in the veterinary clinic. Different types of prophylaxis have been proposed in order to decrease the signs of recurrent UTI's, as well as increase the interval between episodes. Among other proposals for prevention and treatment, there is the use of probiotics. Probiotics are living microorganisms that when administered the right quantity, allow benefits to hosts' health, and also can be used in the prevention, prophylaxis and treatment of various conditions. Thus, this work aims to evaluate the inhibition capacity of genital microbiota bacteria against pathogenic infectious bacteria that cause urinary tract infections and perform a probiotic characterization of bacteria that have a greater inhibitory potential. Dogs of both sexes, with no age restriction, will participate in the study, which will be submitted to clinical, laboratory and imaging examinations to assess health. Genital swabs will be collected for isolation of microorganisms from healthy animals. The pathogens will be provided from urine culture of dogs diagnosed with UTI, confirmed by culture and antibiogram. The identification of microorganisms will be done by PCR. Techniques will be performed to evaluate bacterial competition such as spot on the lawn, radial streak, agar well diffusion, liquid coculture assay and modified cross-streak, from which it is expected the formation of inhibition halos and biochemical changes in the medium, the from the competition between microorganisms isolated from healthy animals and animals with UTI. The results will be interpreted to determine the antimicrobial power of microorganisms from healthy animals in vitro. Afterwards, the probiotic characterization will be performed through the test of resistance to gastric juice, bile salts, multiplication potential, hydrophobicity, formation of hydrogen peroxide and antibiogram. Finally, the sequencing of lactic acid bacteria with satisfactory inhibitory capacity will be performed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MORAES, REINER SILVEIRA; RIBEIRO, DIEGO; TEIXEIRA, MARIA ELOISA; RIBEIRO, GABRIELLA COSTA; FILHO, RAPHAEL LUCIO ANDREATTI; OKAMOTO, ADRIANO SAKAI; GUIMARAES-OKAMOTO, PRISCYLLA TATIANA CHALFUN. Future perspectives of lactic acid bacteria isolated from the urinary tract of healthy dogs: a comment. VETERINARY RESEARCH COMMUNICATIONS, v. 48, n. 5, p. 2-pg., 2024-08-20. (23/08877-0)