EVALUATION OF SYNERGIC AND ANTIBOFILM EFFECTS OS EXTRACTS OF ALECRIM AND JABUTICABA ON Enterococcus faecalis

• Grant number: 23/08140-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2024
• End date: April 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Cláudio Antonio Talge Carvalho
• Grantee: Mariane Takematsu Fujimoto
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Associated research grant(s): 25/04012-0 - FEMS MICRO MILAN 2025 - Microbiology Congress & Exhibition, AR.EXT

Abstract

With the increase of resistant microorganisms in root canals, studies of plant extracts with antibacterial action have increased, aiming at bactericidal alternatives. The aim of this study will be to evaluate the synergistic effect of rosemary and jabuticaba extracts and their anti-biofilm action on Enterococcus faecalis. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) will be determined by microdilution in broth and Fractional Inhibitory Concentration Index and Fractionated Bactericidal Concentration Index by checkerboard microdilution. Subsequently, the effect of the effective concentration of extracts will be evaluated on biofilms formed in microtiter plate wells. The biofilms will be exposed to 2 different concentrations of the extract for 5 min and 24h, with n=12 for each experimental group. As controls, 0.9% saline solution (C+) and 2.5% sodium hypochlorite (C-) will be used, totaling 5 groups for each experimental time. Then, the biofilms will be disaggregated by sonication, the generated suspension will be serially diluted (1:10) and seeded on BHI agar agar. Statistical analysis will be performed using the ANOVA method and Tukey's test (5% significance).