Composition of the telomerase ribonucleoprotein complex of Leishmania major and Leishmania infantum with different genetic backgrounds

• Grant number: 24/00995-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2024
• End date: February 19, 2025
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Maria Isabel Nogueira Cano
• Grantee: Beatriz Cristina Dias de Oliveira
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 18/04375-2 - Studies about the biogenesis and composition of the Leishmania spp. ribonucleoprotein complex and its regulation, AP.TEM

Abstract

Among the Leishmania genus are the causative agents of leishmaniasis, a neglected disease to which there are no effective treatment, control, and prevention. Therefore, it is important to find new parasite-specific therapeutic targets. Telomeres have been recently considered potential targets due to their role in maintaining genome stability and maintenance. The minimal parasite´s telomerase components TERT (telomerase reverse transcriptase) and TER (telomerase RNA) were already described and partially characterized. Preliminary results from our group showed that the parasite orthologue of HSP90, HSP83, forms part of the telomerase complex and probably regulates enzyme activity and telomere length. We intend to identify other components of the complex using Leishmania with different genetic backgrounds. Co-immunoprecipitation and pull-down assays besides of chromatographic and proteomics methods such as mass spectrometry will be used. The validation of the interactions will be confirmed using biochemistry and molecular genetics. The identification of some partners may enhance our comprehension of the biogenesis of the Leishmania ribonucleoprotein complex and help to develop an interaction network that controls the parasite´s telomere length. (AU)