STUDY OF THE INFLUENCE OF HEAT TREATMENT ON COATINGS OF 45S5 BIOGLASS AND BIOK ON ZIRCONIA IMPLANTS: ANALYSIS OF MECHANICAL PROPERTIES AND IN VITRO OISTEOGENESIS

• Grant number: 23/18136-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2024
• End date: March 31, 2025
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Clarissa Merquior Darezzo
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Zirconia implants are considered promising alternatives in rehabilitative treatment, as they address disadvantages associated with titanium implants. BIOK is a bioactive glass with a composition similar to the standard 45S5 bioactive glass, but with the substitution of sodium for potassium. This substitution reduces the crystallization capacity, resulting in more uniform coatings compatible with the substrate. Therefore, coating with BIOK shows great potential for zirconia implants, replacing more invasive processes such as sandblasting, which may reduce implant strength. This study aims to evaluate the effect of the thermal treatment influence on 45S5 bioactive glass and BIOK coatings on zirconia implants in mechanical properties and in vitro osteogenesis. 3Y-TZP zirconia samples will be produced, divided into three groups: polished zirconia (P), zirconia coated with 45S5 bioactive glass (V), and zirconia coated with BIOK bioactive glass (K). Samples produced for characterization tests and in vitro stage will measure 6.0 x 4.0 x 1.6mm³. After production, surface morphology will be analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Average roughness (Ra), surface free energy, and flexural strength analysis will also be performed. Following characterization, samples will undergo in vitro biological tests using mesenchymal cells obtained from rat femurs. Cells will be isolated and plated with samples to assess the influence of biomaterials on osteoblast cell activity and differentiation using cytotoxicity tests, total protein content evaluation, alkaline phosphatase activity, cell morphology analysis, and mineralization nodule formation. Initially, data will be statistically analyzed for normality using an appropriate statistical test. The adopted significance level will be the conventional value of 5%.