MECHANISMS INVOLVED IN RESORPTION AND REPAIR OF REPLANTED TEETH SUBMITTED OR NOT TO EARLY AND DELAYED ORTHODONTIC FORCE

• Grant number: 23/11018-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2024
• End date: April 30, 2026
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Francisco Wanderley Garcia de Paula e Silva
• Grantee: Guido Artemio Maranon Vasquez
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This research proposal aims to investigate the effect of applying orthodontic force on traumatized teeth of rats, after observation periods of 7 and 60 days, on the mechanisms involved in the process of dental repair after trauma. A model of tissue damage resulting from avulsion of upper incisor and treated with immediate replantation will be studied. Wistar rats will be randomly allocated into 8 groups (n = 24) according to trauma application, observation period, and implementation of orthodontic force. The animals will be euthanized after 1, 3, 7 and 14 days of force application. The following analyses will be performed: microtomographic analysis and morphometric analysis using optical microscopy to characterize resorption of the mineralized tissue; histopathological analysis with H&E; assessment of activity of clastic cells by TRAP histoenzymology; evaluation of the activity of MMPs in catabolism regions by in situ zymography; evaluation of the proteomic profile of the periodontal ligament by liquid chromatography-mass spectrometry; evaluation of protein expression patterns through bioinformatics analysis; and, in situ identification of differentially expressed proteins by means of immunohistochemistry. The data will be submitted to statistical analysis adopting a significance level of 5%.