The antimicrobial effect of calcium hydroxide, chlorhexidine digluconate and N-acetylcysteine on strains of P. gingivalis and F. nucleatum

• Grant number: 24/01688-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2024
• End date: April 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Amjad Abu Hasna
• Grantee: Gabriel Manente Cezarino
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

To ensure effective endodontic treatment, the use of intracanal medications is recommended to disrupt biofilms and thereby eliminate residual microbial infections in the root canals, with calcium hydroxide [Ca(OH)2] and chlorhexidine (CLX) being the most commonly used. The objective of this study will be to evaluate the intimicrobial action of Ca(OH)2, CLX and N-acetylcysteine (NAC) and their hybrids, on anaerobic bacteria, including P. gingivalis and F. nucleatum. Antimicrobial evaluation of intracanal medications will involve determining the minimum inhibitory concentration (MIC) and minimummicrobicidal concentration (MMC), assessing antibiofilm activity (MTT test), and evaluatingbiomass using the crystal violet test. All data will undergo normality testing andsubsequently be analyzed using the Kruskal-Wallis test supplemented with the Dunn testfor non-parametric data or one-way ANOVA and Tukey test for parametric data, with asignificance level of ± d 0.05 using GraphPad Prism 6 (La Jolla, CA, USA).