Oxidative stress in dogs with heart disease with myxomatous mitral valve disease

• Grant number: 24/02751-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2024
• End date: December 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Breno Fernando Martins de Almeida
• Grantee: Taís Araújo de Oliveira
• Host Institution: Centro Universitário Faculdades Integradas de Ourinhos. Fundação Educacional Miguel Mofarrej. Ourinhos , SP, Brazil

Abstract

Oxidative stress is a condition that occurs when there is an imbalance in thebetween oxidizing substances and antioxidants, impairing functionsand contributing to the progression of various pathological processes.In addition, there is evidence to suggest that oxidative stress is implicated inpathophysiology and progression of heart disease in dogs. The cardiac work of aDog with heart failure is insufficient to provide normal oxygenationof all cells, which implies a chronic oxygen deficit and therefore ahigh production of free radicals. Hence, the hypothesis that oxidative stressincreases according to the progression of heart disease is a hypothesis to beThe aim of the present study is to evaluate oxidative stressin dogs with heart disease with myxomatous mitral valve disease. Will be selectedTwenty dogs with myxomatous mitral valve disease diagnosed in theVeterinary Medical Center "Roque Quagliato" based on echocardiographic examination andTen more healthy dogs with no alterations in the tests for the composition of the groupControl. Hematological analyses will be performed on an automated counter ofVeterinary cells with differential leukocyte count and morphological analysesin blood smear using light microscopy. The Determinationsbiochemical tests will be performed in an automated photocolorimeter using aof commercial reagents and following the manufacturers' recommendations. Stresswill be determined in an automated photocolorimeter by the evaluation of thetotal antioxidant capacity (CAT) by four methods: trolox equivalent byinhibition of cation reduction ABTS (CAT-ABTS), inhibition of cation reductionPeroxidase-associated ABTS (CAT-ABTS+HRP), ferric reduction (CAT-FRAP) andcupric (CAT-CUPRAC); in addition to the determination of the total oxidizing capacity (TOC),lipid peroxidation by thiobarbituric acid reactive substances (TBARS) andof the antioxidants uric acid and albumin. The variables will be tested for thenormality The differences between the groups will be verified by the non-normal t-tests.paired or Mann-Whitney, being considered significant when p less than 0.05.